猪传染性胃肠炎病毒呼吸道途径侵染后在体内的动态分布  

Dynamic Distribution of Porcine Transmissible Gastroenteritis Virus in Tracheally Inoculated Pigs

作　　者：严懿嘉[1] 邹勇[2] 崔立[1] 钱永清[2] 华修国[1] 

机构地区：[1]上海交通大学农业与生物学院动物医学系,上海201101 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《中国兽医学报》2006年第3期246-247,253,共3页Chinese Journal of Veterinary Science

基　　金：国家科技攻关项目子课题(2004BA717B-01-05)

摘　　要：应用猪传染性胃肠炎病毒(TGEV)分别对25日龄和40日龄的SPF仔猪进行气管内注射攻毒。攻毒后每24h致死1头仔猪,用免疫荧光染色检测各脏器内病毒的消长情况,并采血检测抗体。结果,在25日龄的仔猪中,仅3头出现腹泻症状,且很快耐过;剖检可见不同程度的肺部病理变化;攻毒后24h气管和肺脏样品的IFA呈阳性,抗体效价随着时间的延长而增高。40日龄仔猪临床表现和剖检变化基本正常。所有的胃肠道样品的IFA均为阴性。结论气管内注射后,TGEV可在呼吸系统中增殖,但未能在消化道系统中检出。Specific pathogen-free （SPF） pigs of 25-and 40-day old were tracheally inoculated with TGEV. Although three of fifteen 25-day old pigs developed diarrhea on PID 12-14, they eventually recovered. There were different degree of pathological changes on lung. None of the 40-day old pigs showed any clinical signs. Antibodies against TGEV were detected in all pigs by ELISA. Therefore, there is an age-dependent resistance to pathogenic TGEV infection in pigs. The IFA of samples of trachea and lung were positive, while all samples of intestinal tract had negative response. The result shows that the age-dependant distribution of TGEV is presented in the respiratory system.

关 键 词：猪传染性胃肠炎病毒 免疫荧光抗体 呼吸道感染 病毒分布 

分 类 号：S852.65[农业科学—基础兽医学] S858.28[农业科学—兽医学]