锌中毒对雏鸡外周血T-淋巴细胞的影响(英文)  

Effect of Zinc Toxicity on Peripheral Blood T-lymphocyte in the Broiler

作　　者：崔恒敏[1,2] 赵翠燕[1] 彭西[1] 邓俊良[1] 黎得兵[1] 

机构地区：[1]四川农业大学动物科技学院 [2]四川农业大学都江堰校区,四川都江堰611830

出　　处：《中国兽医学报》2006年第3期310-313,共4页Chinese Journal of Veterinary Science

基　　金：四川省教育厅和科技厅重点项目~~

摘　　要：200只1日龄艾维茵肉鸡随机分为4组,即对照组(每千克日粮含锌100mg)、锌中毒组(每千克日粮含锌1500mg)、锌中毒组(每千克日粮含锌2000mg)、锌中毒组(每千克日粮含锌2500mg),每组均饲喂日粮7周。以流式细胞术和酸性α-醋酸萘酯酶(ANAE)染色法观测外周血T-淋巴细胞的动态变化。结果,3个锌中毒组7周龄时,外周血T-淋巴细胞ANAE阳性率显著低于对照组(P<0.05或P<0.01),同时3个锌中毒组间比较也呈显著差异(P<0.05或P<0.01)。与对照组比较,锌中毒组2至7周龄和锌中毒组6、7周龄CD4+T淋巴细胞数量减少,锌中毒组、组2至4周龄和锌中毒组、组7周龄CD8+T淋巴细胞数量降低,CD4+/CD8+比值3个锌中毒组2、4周龄升高,6、7周龄降低。由此表明,锌中毒可抑制T-淋巴细胞的生成,降低其在外周血中的数量。The experiment was conducted to examine the effect of zinc toxicity on the peripheral blood T-lymphocyte by the method of flow cytometry（FCM） and ANAE. 200 one-day-old Avian broilers were divided into four groups,and fed with the diets as follows ：①control group（Zn 100 mg/kg diet） and ② zinc toxic groups（Zn 1 500 mg/kg diet,zinc toxic group Ⅰ ;Zn2 000 mg/kg diet,zinc toxic group Ⅱ ;Zn 9. 500 mg/kg diet,zinc toxic group Ⅲ ） for seven weeks. The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the three zinc toxic groups than that in control group at 7 weeks of age. CD4^＋ T cell numbers were reduced from 9. to 7 weekds of age in.zinc toxic group Ⅲ and from 6 to 7 weeks of age in zinc toxic group Ⅱ ,as compared with that of control group. The numbers of CD8^＋ T cell decreased at 9. and 4 weeks of age in zinc toxic groups Ⅱ and Ⅲ ,and at 7 weeks of age in zinc grups Ⅰ and Ⅲ. CD4^＋/CD8^＋ ratio was higher at 9. and 4 weeks age,and lower at 6 and 7 weeks of age in zinc toxic groups Ⅰ , Ⅱ and Ⅲ than in control group. The results showed that zinc toxicity would suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations. Potential mechanism underlying these observations are also discussed.

关 键 词：T-淋巴细胞 锌中毒 雏鸡 

分 类 号：S856.9[农业科学—临床兽医学] S858.31[农业科学—兽医学]