转染血管内皮生长因子基因神经干细胞移植对大鼠脑缺血损伤的修复作用  

VEGF-transfected neural stem cells transplantation to striatum of temporary middle cerebral artery occlusion rats

作　　者：朱巍[1] 毛颖[1] 周良辅[1] 汪洋[2] 江永[1] 朱剑虹[1] 

机构地区：[1]复旦大学附属华山医院神经外科,上海200040 [2]复旦大学上海医学院解剖组胚系

出　　处：《中华器官移植杂志》2006年第5期305-308,共4页Chinese Journal of Organ Transplantation

基　　金：2002年上海市科技发展基金重点资助项目(02DJ14025)

摘　　要：目的 探讨转染血管内皮生长因子（VEGF）基因神经干细胞移植对大鼠脑缺血损伤的修复作用。方法 建立大鼠大脑中动脉梗塞（tMCAO）模型，将tMCAO模型随机分为对照组、注射磷酸盐缓冲液（PBS液）的PBS液对照组、接受神经干细胞（NSCs）移植的NSCs组、接受转染VEGF基因的NSCs移植的NSCs-VEGF组，采用立体定向法将相应移植物注射到tMCAO模型的右侧纹状体缺血半暗区。各组移植后2、4、6、8、10、12周进行神经功能损害程度（NSS）评分；移植后7d，采用免疫荧光染色法观察NSCs-VEGF组移植细胞的VEGF基因表达情况；12周时，采用免疫荧光染色法追踪NSCs-VEGF组移植细胞向神经元方向分化情况，并同时行各组移植区周围血管内皮细胞半定量计数。结果 移植后2～12周，NSCs-VEG组的NSS评分均低于其它3组，第12周时的NSS评分与其它3组比较，差异有统计学意义（P〈0．01）；移植后7d，NSCs-VEGF组的移植细胞从移植点向周围迁徙，部分表达VEGF基因；移植后12周，NSCs-VEGF组部分移植细胞分化成神经元，其移植区血管内皮细胞数显著高于其它3组（P〈0．05）。结论 转染VEGF基因的神经干细胞移植对脑缺血所致的损伤具有一定的修复作用。Objective To evaluate the effectiveness of VEGF-transfected neural stem cells （NSCs） transplantation to striatum for amelioration of neural damage induced by cerebral ischemia. Methods Temporary middle cerebral artery occlusion （tMCAO） models were established and randomly divided into control group, PBS transplantation group, NSCs transplantation group （n = 10） and VEGF-secreting NSCs transplantation group （n = 20）. The grafts in each group were transplanted into right striatum respectively 3 days after tMCAO model established. NSS was checked in all groups 2, 4, 6, 8, 10, 12 weeks respectively after transplantation. By using immunofluorescent staining, VEGF expression in transfeeted NSCs was detected 7 days after transplantation. Differentiation of transplanted NSCs was investigated 12 weeks after transplantation. Endothelial cells around the transplantation area were semi-quantitated by using immunohistoehemistry staining. Results NSS in VEGF-seereting NSCs transplantation group was lower than in other three groups from 2-12 weeks. There was significant difference in NSS among the groups 12 weeks after transplantation （P〈0. 01）. VEGF- transfeeted NSCs migrated and expressed VEGF 7 days after transplantation. NSCs survived and migrated, some of them differentiated to neurons and integrated well with hosts＇ eytoarehiteetural corn ponents 12 weeks after transplantation. The number of endothelial cells in VEGF-seereting NSCs transplantation group was significantly more than that in other 3 groups. Conclusion VEGF-transfected NSCs may reduce neural and vascular damage after being transplanted into striatum of tMCAO rats

关 键 词：干细胞移植 血管内皮生长因子类 转染 脑缺血 大鼠 

分 类 号：R651[医药卫生—外科学]