固有型和诱生型一氧化氮合酶mRNA在急性肺损伤后不同时间的表达强度  

THE TIME-COURSE OF CONSTITUTIVE AND INDUCIBLE NITRIC OXIDE SYNTHASE MRNA TRANSCRIPTION IN ACUTE LUNG INJURY

作　　者：孙广运[1] 毛宝龄[2] 张献全[1] 周力平[1] 舒运兵[1] 

机构地区：[1]宜宾市第一人民医院麻醉科,四川宜宾644000 [2]第三军医大学呼吸病研究所

出　　处：《泸州医学院学报》2006年第2期106-109,共4页Journal of Luzhou Medical College

摘　　要：目的:研究大鼠急性肺损伤(ALI)病程中固有型一氧化氮合酶(cNOS)mRNA和诱生型一氧化氮合酶(iNOS)mR-NA表达强度的时相性变化。方法:以静脉注射油酸复制大鼠的ALI模型,用地高辛标记的cNOS和iNOS mRNA的cDNA探针,斑点杂交法分别测定cNOS mRNA和iNOS mRNA表达强度的变化。结果:注入油酸后与对照组比较,cNOS mRNA有下调趋势,5h、12h、1d、3d、5d时分别比对照组降低4.15%、3.37%、4.45%、6.43%、5.79%。注入油酸后与对照组相比,iNOS mRNA表达增强,5h、12h、1d、3d、5d时分别比对照组上升51.90%、60.96%、28.09%、5.79%、4.83%。结论:cNOS mRNA下调提示内皮细胞功能进一步受损,iNOS mRNA上调提示有大量一氧化氮合成,可使ALI进一步加重。Objective： To investigate the time-course of constitutive nitric oxide synthase （eNOS） mRNA and inducible nitric oxide synthase （iNOS） mRNA expression in acute injuried lung tissue of rat. Methods： eNOS and iNOS mRNA activities were measured using dot-blot hybridization labeled with digoxin eDNA probes in oleic acid rat acute hang injury model. Results： eNOS mRNA activity was down-regulated after venous injection of oleic acid ,compared with the controlled group, the relative activity at the time of 5h,12h,ld,3d,and 5d reduced 4.15% ,3.37%,4.45%,6.43%,and 5.79% respectively, iNOS mRNA activity was up-regulated;compared with the controlled group, the relative activity at the time of 5h,12h,ld,3d,and 5d increased 51.90%,60.96% ,28.09%, 5.79% ,and 4.83% respectively. Conclusion： Down-regulated eNOS mRNA may relate to further impairment of vascular endothelial function. Up-regulated iNOS mRNA may relate to large amounts of NO synthesis, which may contribute to further injury of the injured lung.

关 键 词：一氧化氮合酶 MRNA转录 急性肺损伤 固有型 诱生型 

分 类 号：R563[医药卫生—呼吸系统] Q522.2[医药卫生—内科学]