RAPD标记与四川黑山羊表型性状的相关性研究  

Study on the Correlations between RAPD Marker and Phenotypic Traits in the Sichuan Black Goat

作　　者：李力[1] 邱敦莲[1] 黄健[1] 周光明[2] 付昌秀 李强[2] 杨志荣[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室 [2]四川省畜禽改良总站,成都610041

出　　处：《畜牧兽医学报》2006年第5期424-429,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省畜牧业"十五"重点科研项目;四川省科技厅应用基础研究项目

摘　　要：利用16个多态性较强、重复性好的随机引物对四川黑山羊多个种群的成年（1.5～2岁）黑山羊96只个体的血样DNA进行扩增,16个引物共扩增出170条多态的RAPD条带.对给定的标记,将“有带（1）”、“无带（0）”两类个体看作独立样本,采用SPSS软件的Compare Means中的Independent-samples T Test来比较两样本各数量性状平均值的差异.并根据两独立样本间个体表型值的分布筛选与性状真实关联的分子标记.结果表明,在黑山羊群体中共筛选到5个分子标记分别与不同性状相关,其遗传基础可能是该标记与控制性状的QTL或主基因连锁.16 random primers with sufficient polymorphism and reproducible patterns were selected to amplify the blood DNA from 96 adult Sichuan black goat at age of 1.5 to 2 years old. 170 polymorphic RAPD bands were produced with the 16 primers. These bands were recorded in the form of 1 （presence of band） and 0 （absence of band）. To a specific marker, the individuals with different polymorphic bands are treated as different samples. The difference of the average for quan titative traits of the two samples were compared with Independent-samples T Test by using SPSS software. Molecular markers having true correlation with traits were selected according to the distribution of the individual phenotypic value of two independent samples. The results showed that 5 molecular markers in the black goat population were related to different phenotypic traits. It is suggested that this marker may have linkage relationship with the quantitative trait loci or the major gene controlling this trait.

关 键 词：黑山羊 随机扩增多态DNA 体重 体尺 RAPD标记 

分 类 号：S826.2[农业科学—畜牧学]