温氏附红细胞体16S rRNA基因的序列测定和分析  

Sequence and Molecular Characterization of 16S rRNA Gene of Eperythrozoon wenyonii-Hubei Strain

作　　者：付媛[1] 李树清 杜凯[1] 刘琴[1] 周艳琴[3] 刘恩勇 姚宝安[3] 赵俊龙[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070 [2]上海进出口检验检疫局,上海200135 [3]华中农业大学动物医学院,武汉430070 [4]湖北省兽疫防治站,武汉430064

出　　处：《畜牧兽医学报》2006年第5期518-522,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：湖北省畜牧局课题和上海市科委标准专项(04DZ05017)

摘　　要：从自然感染附红细胞体的湖北黄牛无菌采集血液,分离附红细胞体并提取病原基因组,用血营养菌的16SrRNA基因的通用引物进行PCR扩增,得到长约1.5 kb的扩增片段,将其克隆到pMD18-T载体后进行测序和分析。结果表明该片段全长为1 471 bp(GenBank收录号为AY946266),同源性分析表明该序列与Neimark公布的温氏附红细胞体(AF016546)的16S rRNA基因序列同源性达到98.7%,证实该病原为温氏附红细胞体,从分子生物学水平证实了温氏附红细胞体在湖北省的存在。将该序列与5种支原体、14种血营养菌及边缘无浆体等的相应序列进行比较,建立系统发育树,结果表明温氏附红细胞体同边缘无浆体的关系较远,而与肺炎支原体组的亲缘关系较近。The complete 16S rRNA gene of E. wenyonii Hubei strain was determined by PCR. It was sequenced and blasted. The results showed that the sequence was 98.7% identical to that of Neimark published sequence（AF016546）. The 1 471bp complete sequence was compared with the sequence of 5 rnycoplasmas, 14 haemotrophic bacteria and Anaplasma rnarginale available in Gen Bank. The data were analyzed and a phylogenetic tree was established. The results indicated that the hereditary distance of E. wenyonii was close to that of pneumoniae group,and the hereditary distance was far from Anaplasma marginale.

关 键 词：温氏附红细胞体 16S RRNA PCR 序列分析 

分 类 号：S852.62[农业科学—基础兽医学]