稻瘟病菌粗毒素的致病力测定及其对水稻幼苗生理生化的影响  

Determination of Pathogenicity of Rice Blast Crude Toxin and the Influence of Physiological and Biochemical in Rice Seedling

作　　者：陈罡[1] 钟鸣[1] 侯玉柱[1] 刘志恒[2] 马惠[1] 刘少霞[1] 

机构地区：[1]沈阳农业大学/辽宁省农业生物技术重点实验室,辽宁沈阳110161 [2]沈阳农业大学植物保护学院植病系,辽宁沈阳110161

出　　处：《种子》2006年第5期20-23,共4页Seed

基　　金：辽宁省博士启动基金(辽宁省科委2001102061)

摘　　要：本实验用不同浓度的稻瘟病菌粗毒素处理沈农265和辽粳294幼苗,分别对其进行致病力测定,并对其防御酶系、可溶性蛋白、同工酶标记进行了测定分析。结果表明在山口富夫培养液中培养12 d时提取的粗毒素毒性最强,并且对水稻种子萌发,种子胚根、胚芽都有明显的抑制作用;不同浓度毒素对水稻幼苗的POD、SOD,可溶性蛋白均具有一定的诱导作用;同工酶检测表明,粗毒素处理的幼苗POD酶带有明显的变化,而EST没有明显的改变。The seedlings of two rice （ Oryzae satira ） varieties, whose seeds were germinated in the condition of different phytotoxin concentrations, were used as experiment materials. During the process of the experiment, the toxicity bioassay on toxin extracted from pyricularia oryzae under different periods of culture was primarily studied. The results showed that the toxicity reached the top at the twelveth day. The toxin significantly inhibited the germination rate, shoot and root elongation. However, different concentrations of toxin induced the activity of POD and SOD as well as the soluble protein content in seedlings of rice. The results of isozyme assay suggested that there were obvious changes in POD isozyme bands while little changes in EST isozyme bands were detected.

关 键 词：水稻 稻瘟病 防御酶系 可溶性蛋白 同工酶 

分 类 号：S511.01[农业科学—作物学] S435.111.4