铅胁迫对黄瓜幼苗叶片蛋白质的影响  

Effects of Lead Stress on Proteins in Cucumber Seedling Leaves

作　　者：刘素纯[1] 萧浪涛[1] 廖柏寒[2] Annie Zhu jiang Connie Lo 

机构地区：[1]湖南农业大学湖南省植物激素与生长发育重点实验室,湖南长沙410128 [2]湖南农业大学资源环境学院,湖南长沙410128 [3]加州大学洛杉矶分校化学系,加利福尼亚州洛杉矶90024 [4]加州大学洛杉矶分校神经科学系,加利福尼亚州洛杉矶90024

出　　处：《湖南农业大学学报（自然科学版）》2006年第2期141-143,218,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：教育部青年骨干教师基金(2000科教司65号);湖南省教育厅青年基金(99B05)

摘　　要：黄瓜幼苗经不同质量浓度铅处理后,叶片中可溶性蛋白质含量均发生了明显的变化.100 mg/L铅处理时,黄瓜幼苗叶片中可溶性蛋白质含量高于对照值,且随处理时间的延长,与对照比增加的值逐渐减少;500 mg/L和900 mg/L铅处理的叶片中可溶性蛋白质含量随着处理时间的延长表现为下降趋势,并且铅质量浓度越高,随着处理时间的延长,蛋白质含量下降量增加.不同质量浓度铅处理不同时期黄瓜幼苗叶片可溶性蛋白质组分的变化在谱带数和表达量上有明显的差异.The content change of soluble protein in leaves was obviously token place after lead treatment of different mass concentration in cucumber seedling. The content of soluble protein in leaves was higher to control at lead stress of 100 mg/L, the increase amount compared to control showed decrease with the increase of treatment time. The content of soluble protein in leaves showed a downtrend with the increase of treatment times at lead stress of 500 mg/L and 900 mg/L, the decreased content of protein was increased with the increase lead concentration and treatment time. The change of proteomics is evidently different on isoenzymic band numbers and expression at different mass concentration and different period under lead stress. It implies that the synthesis and degradation of protein axe related to the anti-lead toxicity in cucumber seedlings.

关 键 词：黄瓜 铅胁迫 蛋白质 SDS-PAGE电泳 

分 类 号：Q945.78[生物学—植物学]