未衍生化卡托普利的毛细管电泳法测定  

Determination of underivatized captopril by capillary electrophoresis

作　　者：翟海云[1] 朱盛山[1] 陈缵光[2] 李全文[2] 蔡沛祥[3] 莫金垣[3] 

机构地区：[1]广东药学院,广州510224 [2]中山大学药学院,广东广州510089 [3]中山大学化学与化学工程学院,广东广州510275

出　　处：《化学研究与应用》2006年第5期525-528,共4页Chemical Research and Application

基　　金：国家自然科学基金项目(20375049);广东省自然科学基金重点项目(021808)

摘　　要：采用毛细管电泳高频电导法测定了未衍生化的卡托普利。考察了分离检测条件的影响。实验选择10.00mmol/L Tris-5.0mmol/LH3BO3-20.0%CH3OH为电泳介质,在优化条件下,卡托普利的线性范围为1.00μg/mL^200μg/mL,检出限为0.3μg/mL。成功地检测了血清和尿液中的卡托普利。Determination of underivatized captopril by capillary electrophoresis with high frequency conductivity detection was described. The key factors for separation and detection were studied and the best analysis conditions were obtained. In the buffer of 10. 0mmol/L Tris -5.0mmol/L boric acid-20. 0% CH3 OH, captopril in the samples could be separated and detected successfully. Under the chosen conditions,there was a linear relationship between the captopril concentration and the peak area in the range of 1.00μg/mL-200μg/mL. The limit of detection was 0. 3μg/mL. The method was used for analysis of captopril in human serum and urine samples satisfactorily with the recovery of 98. 7-103%.

关 键 词：毛细管电泳 高频电导检测 非接触式电导检测 卡托普利 血清 尿液 

分 类 号：O657.8[理学—分析化学]