高效液相色谱法测定红霉素血药浓度  

A High performance Liquid Chromatographic Method for the Determination of Erythromycine in Plasma

作　　者：张钧寿[1] 任献忠 

机构地区：[1]中国药科大学药剂学教研室

出　　处：《中国药科大学学报》1996年第3期165-168,共4页Journal of China Pharmaceutical University

摘　　要：建立了ＨＰＬＣ测定红霉素血药浓度的新方法。以峰面积定量，浓度与峰面积具有良好相关性（ｒ＝０．９９８），线性范围０．４～４．０μｇ／ｍｌ，最低检测限０．１μｇ／ｍｌ。浓度为０．４，１．６，３．２μｇ／ｍｌ的回收率均大于９３．４％，三个浓度下测定的日内差及日间差（ＲＳＤ）分别为１．２３％，１．４９％，１．６７％和２．１８％，３．２６％，４．１７％，与微生物法测定结果无显著差异。A high performance liquid chromatographic method for the determination of erythromycine in plasma is described. The method comprises the extraction from the plasma samples, and the base catalyzed decyclisation. The reaction product was detected by a reversed phase HPLC system installed with UV measurements at 235.5±1 nm. The linear range of concentration was 0.4 to 4.0 μg/ml with the correlation coefficient 0.998. The minimal determinable concentration was 0.1 μg/ml. At the three concentrations of 0.4, 1.6, 3.2 μg/ml, the recoveries were above 93.4% ; the intra and inter day variation coefficient were 2.18%,3.26%,4.17% and 1.23% , 1.49% ,1.67% respectively. The result of the microbiologic method agreed with that of the HPLC method.

关 键 词：红霉素 高效液相色谱 血药浓度 

分 类 号：R978.15[医药卫生—药品]