硒缺乏致发雏鸡贫血机理的研究  

Study on the Pathogenesis of Anemia in Chicken with Selenium Deficiency

作　　者：仝宗喜[1] 王永才[1] 杨凤兰[1] 康世良[2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《河南农业大学学报》2006年第2期168-172,共5页Journal of Henan Agricultural University

基　　金：黑龙江省自然科学基金资助项目(2002-05)

摘　　要：为探讨雏鸡硒缺乏时发生贫血的机理,选用1日龄健康海兰褐雏鸡120只,随机分成2组,每组60只,第1组为试验组,饲喂低硒饲料(硒含量为0.014 mg.kg-1);第2组为对照组,饲喂全价饲料(硒含量为0.375 mg.kg-1).自由采食与饮水.分别于雏鸡10,20,30,40 d时每组选10只鸡心脏采血1 mL,离心分离血清,应用放免法测定血清中的细胞因子和甲状腺激素的含量.结果表明,硒缺乏组雏鸡血清中T3,FT3,IL-1和EPO含量较正常对照组有较大幅度降低,差异极显著(P<0.01);TNF,T4和FT4含量较正常对照组有较大幅度升高,差异极显著(P<0.01).硒缺乏雏鸡发生了3种类型的贫血.One-day old chicken （ n = 120） were randomly divided into 2 groups （ n = 60 in each group） ,to investigate the pathogenesis of anemia in chicken with selenium deficiency. The chicken in the first group, the experimental group, were fed with selenium-deficiency diet（selenium 0.014 mg·kg^-1）; The ones in the second group, the control group, were fed with the basal diet （ selenium 0. 375 mg·kg^-1 ）, which fed and drank freely. 1 mL of blood sample from heart （ n = 10 in each group） were collected at day10,20,30 and 40 after feeding and serum separated with centrifuge. The contents of cytokines and thyroid gland hormone in serum were determined by the method of RIA. The results showed that the content of T3, FT3, IL-1 and EPO in the experimental group was significantly lower than that in the control group （ P 〈 0.01 ） and the content of TNF,T4 and FT4 was significantly higher than that in the control group（ P 〈 0.01）. It can be concluded that the chicken with selenium deficiency showed three kinds of anemia.

关 键 词：硒缺乏 雏鸡 贫血 血清 

分 类 号：S831.7[农业科学—畜牧学]