PCR检测婴儿利什曼原虫无症状感染的研究  

Study on PCR Method for Detecting the Asymptomatic Infection of Leishmania infantum

作　　者：高春花[1] 汪俊云[1] 杨玥涛[1] 包意芳[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组,织疟疾,血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第2期92-96,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：世界卫生组织TSA基金资助项目(No.1079946)~~

摘　　要：目的建立适合检测我国婴儿利什曼原虫无症状感染的PCR方法。方法选择6种常用于诊断内脏利什曼病的PCR引物(RV1-RV2、K13A-K13B、MC1-MC2、174-798、Pia3-Pia4和DBY-Ajs31),以培养的甘肃人株利什曼原虫前鞭毛体种植人抗凝全血抽提的DNA为模板,确定了这6种PCR引物检测我国婴儿利什曼原虫的最适条件,并比较其检测的敏感性和特异性。选用两种敏感性和特异度均佳的引物对采自利什曼病疫区100份无利什曼病症状居民的静脉血进行检测。结果6种PCR引物检测的特异性均达到100%,而检测的敏感性各异,检测到的原虫数目从0.1～1000条原虫/ml,其中引物RV1-RV2(0.1个原虫/ml血)和K13A-K13B(1个原虫/ml血)敏感性较高。这两对引物对100份无症状居民血的阳性检出率分别为33%(33/100)和30%(30/100)。结论引物RV1-RV2和K13A-K13B适于检测我国婴儿利什曼原虫无症状感染。在我国甘肃动物源性利什曼病疫区,人群利什曼原虫无症状感染率颇高。Objective To establish PCR method for the detection of the asymptomatic infection of Leishmania infantum. Methods Six primer pairs were selected for detecting Chinese strain of L. infantum by optimizing conditions which affect amplification. Their sensitivity and specificity were compared by using DNAs extracted from human blood seeded with cultured L. infantum promastigotes （MHOM/CN/86/GS） as template. Blood samples of the inhabitants without symptoms of visceral leishmaniasis in the endemic area were analyzed with two selected primer pairs with good sensitivity and specificity. Results The specificity of all six primer pairs reached 100%, and the sensitivity varied among the primer pairs. The primer pairs RV1-RV2 （0.1 parasite/ml blood） and K13A-K13B （1 parasite/ml blood） were most sensitive. Leishmania DNA was detected in 33% （33/100） and 30% （30/100） human blood samples by RV1-RV2 and K13A-K13B primer pairs respectively. Conclusion This study suggests that RV1-RV2 and K13A-K13B primer pairs are suitable in detecting the asymptomatic infection of L. infantum, and the prevalence of the asymptomatic infection is high in human population in the endemic area.

关 键 词：婴儿利什曼原虫 无症状感染 PCR 

分 类 号：R531.6[医药卫生—内科学]