胶体金免疫渗滤法检测日本血吸虫病血清抗体的研究  

Antibody Detection in Sera of Patients with Schistosomiasis Japonica by Dot Immunogold Filtration Assay

作　　者：许静[1] 严自助[1] 张瑞鉴 冯婷[1] 王强[1] 钱翠珍[1] 吴晓华[1] 朱丹[1] 郭家钢[1] 周晓农[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,上海200025 [2]福建省三明博峰生物科技有限公司,三明365000

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第2期146-147,155,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：应用建立的胶体金免疫渗滤法检测日本血吸虫病血清抗体,并以快速酶联免疫吸附试验(F-ELISA)方法检测作平行对照。现场和实验室试验表明,胶体金渗滤法与F-ELISA方法在敏感度和特异度的差异无统计学意义(P>0.05),两法具有较高的一致性。但胶体金免疫渗滤法操作简便快速,无特殊要求,在临床和现场防治工作中具有比较广泛的应用前景。Serum antibody of schistosomiasis patients was detected by dot immunogold fihration method （DIGFA） in laboratory and field, and F-ELISA was used as control. The resuhs showed that there was no significant difference between these two assays in sensitivity and specificity（P〉0.05）, with a high coincidence. DIGFA is easy to operate and may deserve a wide application in the diagnosis of schistosomiasis.

关 键 词：胶体金免疫渗滤法 快速酶联免疫吸附试验 日本血吸虫病 抗体 

分 类 号：R532.21[医药卫生—内科学]