日本血吸虫pcDNA3.1(-)/Sj20.8真核表达及其对小鼠保护性免疫作用  

Induction of Protective Immunity Against Schistosoma japonicum By pcDNA3.1/Sj20.8 Nucleic Acid Vaccine in BALB/c Mice

作　　者：曾桥[1] 肖建华[1] 万志刚[1] 廖力[1] 张愉快[1] 刘传爱[1] 杨秋林[1] 

机构地区：[1]南华大学病原生物研究所,衡阳421001

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第2期153-155,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：湖南省卫生厅重点项目基金的资助(No.2001-Z05)~~

摘　　要：为检测日本血吸虫新基因Sj20.8作为核酸疫苗能否诱导小鼠产生保护性免疫作用,用PCR法扩增Sj20.8基因序列,构建重组质粒pcDNA3.1(-)/Sj20.8,肌肉注射免疫小鼠后检测发现,Sj20.8能在小鼠肌肉中短时间存在并有微弱表达,但未能诱导小鼠产生一定水平的保护性免疫。Sj20.8 gene was amplified by PCR and inserted into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/Sj20.8, which was then injected into the quadriceps femoris of the BALB/c mice. Results showed that the Sj20.8 antigen was low expressed in the local tissue of the mice, and was not able to significantly reduce eggs in the liver than in the control mice.

关 键 词：日本血吸虫 CDNA文库 基因 Sj20.8 

分 类 号：R532.21[医药卫生—内科学] R392.11[医药卫生—临床医学]