自体静脉移植术后血管平滑肌细胞内c-fos、c-myc和c-jun的表达  

The study on the expression of immediate-early response gene c-fos,c-myc,c-jun,in VSMC after autogenous vein grafts

作　　者：王永军[1] 张卫国[2] 杨健[1] 王明民[3] 崔瑞耀[4] 徐万群[4] 于文钢[4] 周滨 

机构地区：[1]青岛海慈医院心内科,山东青岛266033 [2]新乡医学院第一附属医院心内科,河南新乡453100 [3]青岛海慈医院急诊外科,山东青岛266033 [4]青岛大学医学院病理生理学教研室,山东青岛266021 [5]枣庄市第三人民医院,山东枣庄277100

出　　处：《新乡医学院学报》2006年第3期235-237,共3页Journal of Xinxiang Medical University

基　　金：山东省教育委员会资金资助项目(j99k-52)

摘　　要：目的探讨自体静脉移植术后血管平滑肌细胞(VSMC)早期应答基因的变化。方法利用显微外科技术制作64只大鼠颈动脉自体静脉移植模型,采用免疫组织化学染色方法观察VSMC增殖与c-mycc、-fosc、-jun的变化。结果手术后2 h即可出现c-myc、c-fos、c-jun阳性表达,且c-fosc、-jun于移植后6 h达到高峰,c-myc于手术后1周达到高峰。结论c-mycc、-fosc、-jun表达状态与VSMC的增殖状态密切相关,对VSMC的增殖起到调控作用,尤其是始动阶段。Objective To investigate the changes of immediate- early response gene （c-fos, c-myc, c-jun） in vascular smooth muscle cells （VSMC） after autogenous vein grafts. Methods Autogenous transplanting vein models for 60 rats were developed. The changes of vascular VSMC proliferation and immediate- early response, se gene were detected by using immunohistochemstry technique.Results Expression of c-fos, c-jun, c-myc was observed at 2 h after the grafting. Expression of c-fos, c-jun peaked at 6 h after the grafting and c-myc at 1 week.Conclusion Expression status of c- fos, c-myc, c-jun is closely related to VSMC proliferation and regulates for it, particularly in the initial stage.

关 键 词：血管移植 再狭窄 早期应答基因 术后 C-FOS C-MYC C-JUN 

分 类 号：R654.3[医药卫生—外科学]