巴戟天水溶性多糖分离纯化的研究(英文)  

Isolation of Water Soluble Polysaccharides from Morinda officinalis How

作　　者：何传波[1] 陈玲[1] 李琳[1] 郭祀远[1] 李冰[1] 吴少雄[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]昆明医学院,云南昆明650031

出　　处：《云南农业大学学报》2006年第3期320-323,328,共5页Journal of Yunnan Agricultural University

基　　金：广东省科技攻关项目资助(2003C104025)

摘　　要：采取水提醇沉工艺获得了巴戟天粗多糖MOP，用离子交换柱层析方法对粗多糖进行了分级纯化，考察了不同层析缓冲液条件下，巴戟天多糖的动态吸附情况。结果表明：较高pH值和低离子强度的缓冲液有利于巴戟天多糖的吸附，确定层析用缓冲液pH值为8．0—8．5，Tris浓度为0．02mol／L，流速1．5mL／min；在此层析条件下，采用阶段梯度洗脱方式，在NaCl浓度为0，0．1和0．5mol／L时分别获得巴戟天多糖组分MOHP—Ⅰ，MOHP—Ⅱ，MOHP-Ⅲ，用NaOH洗脱得到了MOHP—Ⅳ组分。The water soluble polysaccharidcs were extracted with hot water and subsequently precipitated with ethanol from Morinda officinalis How （MOP）. And the crude polysaccharides were then isolated by anion-exchange chromatography. Effect of different chromatography conditions on adsorption and elution of MOP were discussed, and the results showed that, the adsorbent exhibited better adsorption performance for MOP under higher pH and lower ion strength of buffer solutionn. Suitable column chromatography condition obtained was ： Tris concentration of buffer 0.02 mol/L, pH of buffer 8.0 - 8.5 and flow rate 1.5 mL/min. Four polysaccharide fractions, named MOHP- Ⅰ , MOHP-Ⅱ , MOHP-Ⅲ and MOHP-Ⅳ respectively were obtained using step-wise elution manner.

关 键 词：巴戟天 多糖 分离 影响 

分 类 号：Q539[生物学—生物化学]