云南铁角蕨与泸山铁角蕨的关系:来自叶绿体rbcL、trnL-F和rps4-trnS序列的证据  

Relationship of Asplenium yunnanense and A. lushanense inferred from the sequence analysis of chloroplast rbcL, trnL-F and rps4-trnS

作　　者：李春香[1] 陆树刚[2] 

机构地区：[1]中国科学院南京地质古生物研究所现代古生物学和地层学国家重点实验室,南京210008 [2]云南大学生态学与地植物学研究所,昆明650091

出　　处：《植物分类学报》2006年第3期296-303,共8页Acta Phytotaxonomica Sinica

基　　金：国家自然科学基金项目(40302003;30370116);中国科学院知识创新工程重要方向项目(KZCX2-SW-130)~~

摘　　要：云南铁角蕨Aspleniumyunnanense与泸山铁角蕨Aspleniumlushanense的分类是一个悬而未决的问题。本文对云南铁角蕨与泸山铁角蕨及近缘类群的叶绿体rbcL基因和trnL-F、rps4-trnS基因间隔区序列进行PCR扩增和序列分析;并与已经发表的铁角蕨属植物的相应序列进行比较,发现云南铁角蕨与泸山铁角蕨的rbcL基因和trnL-F、rps4-trnS基因间隔区序列之间均未表现出差异,因此叶绿体DNA序列的证据不能将云南铁角蕨与泸山铁角蕨分开,从而从分子系统学方面证明云南铁角蕨与泸山铁角蕨是一对亲缘关系非常近的物种。The relationship between Asplenium yunnanense Franch. and Asplenium lushanense C. Chr. has long been in dispute, and here was evaluated based on the sequence analysis of chloroplast rbcL gene, trnL-F and rps4-trnS intergenic spacers. No sequence difference in the studied chloroplast DNA regions was found between Asplenium lushanense and A. yunnanense, but some nucleotide differences in the corresponding DNA fragments were found among other Asplenium species. So, A. yunnanense and A. lushanense cannot be distinguishable in terms of chloroplast DNA sequences, and the very close relationship between them is further affirmed based on the evidence from molecular data.

关 键 词：云南铁角蕨 泸山铁角蕨 叶绿体DNA序列 亲缘关系 

分 类 号：Q941.3[生物学—植物学] Q949