MTT法测定不同铁浓度对体外Caco—2细胞生长的影响  

The Effect of Caco——2 Cell Growth Curve In Different Iron Concentrations With MTT

作　　者：孔亮[1] 罗海吉[1] 卢晓翠[1] 杨真[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院营卫系,广州510515

出　　处：《微量元素与健康研究》2006年第3期4-5,共2页Studies of Trace Elements and Health

摘　　要：目的:探讨不同浓度铁剂对体外Caco—2细胞系生长的影响,为研究体内铁稳态调节作用机理提供实验参考。方法:利用6种不同铁浓度的NaFeEDTA试剂作用Caco—2细胞,MTT比色法测定细胞生长情况,绘制生长曲线。结果:随着培养液铁浓度的增高,细胞生长呈上升趋势,尤其在培养液铁浓度>400μmol/L时,细胞生长呈显著性增长(P<0.05)。结论:高浓度铁剂可促进细胞生长、增殖,而大量细胞繁殖与铁代谢调节及铁稳态的维持有着密切的关系。Objective： To study the growth of in vitro Caco- -2 cell in different iron concentrations, in order to provide the reference of iron homeostasis mechanism of action. Method： Caco-2 cell monolayers in vitro treated by NaFeEDTA at six kinds of concentrations, determined the cell growth conditions with MTT chromatometry. Results： The growth were upgraded by the concertrations adding, At the time of iron concertration 〉 400 μmol/L in culture fluid, growth curve reaching had difference reached statistical significance （P 〈 0.05 ） . Conclusions： Homoconcertration chalybeatus could enhance the cell growth and reduplication, It correlated with the iron metabolism and homeostatic on cellular levels.

关 键 词：MTT比色法 CACO-2细胞 铁 生长曲线 

分 类 号：O657[理学—分析化学]