鼠疫DNA疫苗pVAX1/F1-V安全性初步研究  

A preliminary study on safety of plague DNA vaccine in mice

作　　者：郭习勤[1] 王栋[2] 王希良[2] 蒋会婷[1] 黄顺军[2] 韩岳[2] 何维明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《西北农林科技大学学报（自然科学版）》2006年第5期31-34,共4页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性,试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度,并以小鼠为动物模型进行了质粒pVAX1／F1-V的急性毒性和长期毒性试验,用PCR方法检测外源基因在小鼠组织中的分布情况,用ELISA、EIISPOT方法检测了其自身的免疫反应。结果表明,提取的质粒pVAX1/F1-V未检测到核酸及菌体蛋白污染;注射初期质粒pVAX1/F1-V 主要分布于注射部位的肌肉组织,其他组织有散在分布;小鼠接种4周后,质粒pVAX1/F1-V仅分布于注射部位。小鼠注射pVAX1/F1-V质粒后未产生明显的毒副反应及自身免疫损伤,也未检测到自身抗体。初步证明鼠疫DNA 疫苗pVAX1/F1-V安全性良好,为鼠疫菌新型DNA疫苗的应用奠定了基础。To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague,the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method;pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection. Then, long term toxicity and acute toxicity study were conducted on mice mode,antinuclear antibodies and selfimmunoreaction were analyzed by ELISA and ELISPOT method. Results showed that plasmid DNA was pure and can be detected primarily in the muscle at the site of injection,where it remained for 4 weeks. Repeated intramuscular injections of DNA vaccine pVAX1/F1-V showed no adverse effects on behaviors, weight,and no evidence of autoimmune mediated pathology and antinuclear antibodies were observed in the mice. These results suggested that the DNA vaccine pVAX1/F1-V was safe and well tolerated.

关 键 词：鼠疫耶尔森氏菌 毒性试验 DNA疫苗 安全性 

分 类 号：R516.803[医药卫生—内科学] R392-33[医药卫生—临床医学]