促生菌CH1诱导黄瓜对猝倒病抗性的研究  

Studies on the Resistance to Cucumber Damping-off Induced by Plant Growth-promoting Rhizobacteria CH1

作　　者：梁建根[1] 张炳欣[1] 陈振宇[1] 喻景权[2] 

机构地区：[1]浙江大学植物保护系 [2]浙江大学园艺系,杭州310029

出　　处：《园艺学报》2006年第2期283-288,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目(30230250)

摘　　要：采用分根法研究了植物生长促生菌CH1诱导黄瓜对猝倒病的抗性以及黄瓜植株经CH1处理后根部与叶部组织的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性变化。结果表明,促生菌CH1提高了黄瓜的成苗率,处理后的28d,猝倒病相对防效高达78.64%,推测促生菌CH1使黄瓜产生的诱导抗性是其防病的主要原因。黄瓜经CH1处理并同时接种腐霉菌后,处理根部与未处理叶部组织中SOD、POD、CAT、PPO和PAL活性都明显高于其它处理,其根部这些酶的活性分别在处理后3、3、5、5和5d时出现高峰,叶部酶活性变化基本与根部的相吻合。表明这些酶活性的提高与CH1诱导的黄瓜对猝倒病抗性可能有一定的相关性,而且CH1对这些酶活性的诱导具有系统性。Using root-splitting method, the resistance to cucumber damping-off induced by plant growth promoting rhizobacteria CH1 and the changes in activities of superoxide dismutase （SOD）, peroxidase （POD）, catase （CAT）, polyphenol oxidase （PPO） and phenylalanine ammonia-lyase （PAL） in cucumber roots and leaves pretreated with PGPR CH1 and inoculated by Pythium aphanidermatum were studied. The resuits showed that PGPR CH1 could enhance the surviving seedling of cucumber, control efficacy reached 78.64% after CH1 treatment at 28 d, which indicated that induced resistance was the major factor for CH1 to control the cucumber damping-off. The activities of SOD, POD, CAT, PPO and PAL in the roots treated by CH1 and the leaves nontreated by CH1 increased higher than those of other treatments to some extent and reached their maximum on 3rd, 3rd, 5th, 5th and 5th in roots, respectively, the activities of these protective enzymes in leaves were accorded with those of enzymes in roots. The experimental results suggested that the increased activities of SOD, POD, CAT, PPO and PAL induced by CH1 against Pythium aphanidermatum are most likely to be associated with the development of ISR in cucumber. Moreover, the resistance induced by CH1 is systemic.

关 键 词：黄瓜 植物生长促生菌 保护酶 黄瓜猝倒病 诱导抗性 

分 类 号：S642.2[农业科学—蔬菜学] S476[农业科学—园艺学]