变叶海棠遗传多样性的AFLP分析  

Genetic Diversity of Malus toringoides(Rehd.) Hughes Based on AFLP

作　　者：石胜友 成明昊[2] 梁国鲁[2] 郭启高[2] 李晓林[2] 周志钦[2] 

机构地区：[1]湖北省宜昌市林业局,宜昌443000 [2]西南大学园艺园林学院

出　　处：《园艺学报》2006年第2期381-384,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目(30471206)

摘　　要：用8对引物对变叶海棠30个不同的变异类型进行了AFLP分析,共扩增出656条带,其中多态性带为568条,平均每对引物扩增出71条多态性带,多态性带百分率为86.59%,各变异类型多态性比例介于0.3582～0.6296之间;依据简单匹配系数对AFLP扩增结果进行UPGMA聚类,30个变异类型在0.68水平上分为12个类群;该试验从DNA水平上探明了变叶海棠的遗传多样性是变叶海棠与陇东海棠和花叶海棠产生渗入杂交形成的。The study is mainly on the genetic diversity of Malus toringoides （Rehd.） Hughes by applying AFLP. 658 bands were obtained with 8 pairs of AFLP primers based on 30 variant types, and among them 568 （86. 59% ） were polymorphic with an average of 71 bands for per AFLP primer, the ratio of polymorphic rate varied from 0. 3582 to 0. 6296. UPGMA cluster analysis based on simple matching coefficient （SM） divided the thirty variant types into twelve groups at O. 68 level. At DNA level, the results showed that the rich diversity in M. toringoides （ Rehd. ） Hughes was derived from the introgression of M. toringoides （ Rehd. ） Hughes with M. transitoria Schneid. and M. kansuensis Rehd.

关 键 词：变叶海棠 AFLP 遗传多样性 聚类分析 

分 类 号：S661.4[农业科学—果树学]