大鼠肺动脉平滑肌细胞的培养  

Culture of Rat Pulmonary Artery Smooth Muscle Cell

作　　者：李智[1] 张庆利[1] 丛华[1] 郭政东[1] 

机构地区：[1]中国医科大学基础医学院药理教研室

出　　处：《中国医科大学学报》1996年第3期221-224,共4页Journal of China Medical University

基　　金：国家"八五"科技攻关项目

摘　　要：大鼠肺动脉壁薄，平滑肌细胞数少，经人工继代培养后细胞往往显示与在体内时不同的反应，故用荧光分光光度计检测该处细胞内钙难于实现。共焦激光显微钝可以测试一个细胞内钙浓度的动态变化，要求所测试细胞必须是非收缩细胞，且较牢固地粘附在培养皿上。本法应用０．２％Ｃｏｌｌａｇｅｎａｓｅ，０．０１２５％Ｅ－ｌａｓｔａｓｅ，０．１２５％Ｔｒｙｐｓｉｎ分离大鼠肺动脉平滑肛细胞进行原代培养，经间接荧光抗体染色法证明９５％以上为平滑肌细胞。此法培养的细胞可用于共焦激光显微镜测试细胞内Ｃａ（２＋）等浓度的动态变化。he rat pulmonary artery cells are thin-walled, of small quantity, and they usually show differentresponses from those in vivo after being cultured sccondarily, therefore it is difficult to detect the intra-ceIlular Ca(2+) concentration of them by using fluorescence spectrophotometer, ACAS(570) can be used todetect the dynamic changes of intracellular Ca(2+) concentration of single smooth muscle cell which mustbe of non-contractile type and adhere to the cultural plate firmly. The primary culture of isolated ratpulmonary artery smooth muscle cells was performed by using 0. 25%Collagenase,0. 0125%Elastase,and 0. 125%Trypsin, more than 95%of the isolated cells were tested as smooth muscle cells by indi-rect fluorescence antibody staining method, The cells cultured by this method can be used to detect thedynamic changes of intracellular Ca(2+) concentration by cofocal laser microscope。

关 键 词：肺动脉 平滑肌细胞 荧光抗体染色 细胞培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]