新型双层皮肤组织工程支架的构建  

Fabrication of a Bilayer Scaffold for Skin Tissue Engineering

作　　者：关嫚[1] 任磊[1] 吴婷[1] 孙莉萍[1] 张其清[1] 

机构地区：[1]厦门大学化学化工学院化工系厦门大学医学院生物医学工程研究中心,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第3期379-382,共4页Journal of Xiamen University：Natural Science

基　　金：福建省重大科技计划项目(2003006);"863"计划(2002AA326040);科技创新资金项目(3502Z20041029);天津市科技发展计划应用基础研究重点项目(033803011)资助

摘　　要：模拟天然皮肤的真、表皮结构,以胶原和壳聚糖为主要原料构建皮肤组织工程双层支架.将固含量0.8%(mass)的胶原-壳聚糖溶液冻干后压膜,经戊二醛交联后再冻干形成双层支架的底层,然后注入固含量0.2%(mass)的胶原-壳聚糖溶液,经冻干、交联、再冻干即得结构较稳定的双层复合海绵支架.在双层支架上培养的小鼠成纤维细胞贴壁生长正常.本研究构建的胶原-壳聚糖双层支架具有良好的结构稳定性和细胞相容性.To simulate the natural skin composed of dermis and epidermis,a bilayer collagen-chitosan scaffold was developed. The bilayer collagen-chitosan scaffold was prepared by lyophilization of 0.8% （mass） collagen-chitosan solution, then it was pressed by 15 kg weight for 24 h,and cross-linked by 0.2% （mass） glutaraldehyde at 4℃for 24 h. After the scaffold was freeze-dried,0.2%（mass） collagen-chitosan solution was infused,freeze-dried,cross-linked by 0.2% （mass） glutaraldehyde at 422 for 24 h, freeze-dried again. The microstructure and cytocompatibility of the scaffolds were investigated. The contraction ratio enhanced with the increase of content of collagen-chitosan solution. The contraction ratio was reduced after 24 h of pressure. The surface layer of the scaffold prepared by 0.8 % （mass） collagen-ehitosan solution improved the adhesion of cells. The inner layer of the scaffold prepared by 0.2 % （mass） collagen-chitosan solution promoted the proliferation of cells. The bilayer collagen-chitosan scaffold has good cytocompatibility and structure stability. Thus, it is suitable as scaffold for skin tissue engineering.

关 键 词：组织工程 皮肤 胶原 壳聚糖 

分 类 号：O63[理学—高分子化学] R318.08[理学—化学]