从深海沉积物中筛选具有抗菌、抗肿瘤活性的海洋真菌  

Antimicrobial and Antitumor Activities of Marine Fungi Isolated from Sediment in Deep Sea

作　　者：熊枫[1] 郑忠辉[1] 黄耀坚[1] 宋思扬[1] 徐庆妍[1] 苏文金[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第3期419-423,共5页Journal of Xiamen University：Natural Science

基　　金：国家863计划(2003AA624010);福建省科技计划项目(2004N003)资助

摘　　要：从西太平洋近赤道区采集到的18个深海沉积物样品中分离到83株海洋真菌,采用滤纸片琼脂扩散法和MTT法分别对其发酵液乙酸乙酯抽提物的抗菌、抗肿瘤活性进行筛选.结果显示,有37株菌株对至少一种指示菌有抑制作用;有29株菌株对KB或Raji肿瘤细胞具有显著的抑制活性,分别占总供测菌株的44.6%和34.9%.在抗肿瘤活性菌株中,WP-M-1、DY-G-2、DY-C-2、WP-K-3和WP-Q-2等5株菌具有很高的活性,其发酵液乙酸乙酯抽提物对Raji细胞的IC50分别为5.000、1.064、2.796、1.920、0.520μg/mL.研究结果表明,海洋沉积物来源的真菌中蕴藏着丰富的抗菌及抗肿瘤活性代谢产物产生菌,是开发抗菌和抗肿瘤药物的宝贵资源.Marine organisms are a rich source of structurally novel and biologically active metabolites. So far, many chemically unique compounds of marine origin with different biologically activity have been isolated and a number of them are under investigation and/or are being developed as new pharmaceuticals. In this report, we describe the screening of ethyl acetate extracts of eighty-three strains of marine sediment fungi,collected from west of the Pacific Ocean beside the equator, for antimicrobial and antitumor activities. As a result, thirty-seven strains showed inhibition against one or more of those tested microorganism, namely EC, SA, BS, CA, ANS,while twenty-nine strains of fungi demonstrated antitumor activity againnst KB cell or Raji cell respectively. The ratio of such activity fungi to total tested fungi was 44. 6 %, 34.9 %. Furthermore,five fungal extracts, namely WP-M-1 ,DY-G-2,DY-C-2,WP-K-3 and P-Q-2,were chosen to study the IC50 against Raji cell, the value of them was 5. 000,1. 064,2. 796,1. 920,0. 520μg/mL respectively. These results indicated that marine sediment fungi would be a promossing resourse of antimicrobial and antitumor medicament.

关 键 词：海洋沉积物 海洋真菌 抗菌活性 抗肿瘤活性 

分 类 号：Q939[生物学—微生物学]