RP-6启动子连接的IPT基因的双元载体构建  

The Construction of a Plant Binary Vector pCAMBIA130-pRP-6-IPT-Nos for Expressing IPT Gene in Developing Rice Seed

作　　者：张红心[1] 李晓星[1] 苏勇波[1] 黄维雪[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第3期432-435,共4页Journal of Xiamen University：Natural Science

基　　金：福建省青年科技人才创新项目(2005J004)资助

摘　　要：以含有IPT基因的中间载体pSG516为基础,采用酶切的方法获得IPT-Nos核酸片段,以水稻品种9311为材料,采用PCR的方法克隆出种子中特异表达的醇溶谷蛋白RP-6基因启动子,并将此启动子连接到pCAMBIA1300上,构建pCAMBIA1300-pRP-6载体,将IPT-Nos核酸片段插入到pCAMBIA1300-pRP-6,构建了pCAMBIA1300-pRP-6-IPT-Nos双元表达载体.In order to obtain transgenic rice which express exogenous IPT gene in developing seed and to further investigate the function of cytokinin in rice seed development, we constructed the binary vector pCAMBIA1300-pRP-6-IPT-Nos. Firstly IPT-Nos fragment was obtained from pSG516 by cutting PSG516 plasmid with Nco I and Spe I restriction enzymes. Secondly,developing endosperm RP-6 gene specifically expressed in developing endosperm was selected and it＇s promoter （pRP-6） was isolated from rice genomic DNA （cultivar 9311） by using PCR amplification. Thirdly pRP-6 was inserted into pCAMBIA1300, with pCAMBIA1300-pRP- 6 obtained. Finally,plant expression vector pCAMBIA130-pRP-6-IPT-Nos was constructed after inserting IPT-Nos fragment into pCAMBIA1300-pRP-6.

关 键 词：pRP-6 IPT pCAMBIA1300-pRP-6-IPT-Nos 特异表达 

分 类 号：Q78[生物学—分子生物学]