阿托伐他汀对人脐静脉内皮细胞增殖活性的影响  

Effect of atorvastatin on the human umbilical vein cells

作　　者：焦力群[1] 鲍圣德[1] 莫大鹏[1] 高鹏[2] 陈左权[2] 

机构地区：[1]北京大学第一医院神经外科,100034 [2]同济大学脑血管疾病中心

出　　处：《中国脑血管病杂志》2006年第5期201-203,210,共4页Chinese Journal of Cerebrovascular Diseases

基　　金：国家自然科学基金资助项目(30500519)

摘　　要：目的观察阿托伐他汀对人脐静脉内皮细胞增殖(humanumbilicalveinendothelialcells,HUVEC)及超微结构的影响。方法将HUVEC分为3组进行培养,包括正常对照组、重组人白细胞介素6(rhIL6)刺激组和阿托伐他汀组。通过MTT法测定各组细胞的增殖活力,并用透射电镜观察各组细胞超微结构的改变。结果①正常对照组、rhIL6组及阿托伐他汀组的吸光度(A)分别为0.172±0.014、0.257±0.058及0.184±0.021;②rhIL6作用下,HUVEC粗面内质网上核糖体较对照组明显增多,而阿托伐他汀可抑制rhIL6对HUVEC的这种超微结构改变。结论阿托伐他汀能有效抑制rhIL6,进而阻止HUVEC增殖。ObjecUve To study the effect of atorvastatin on proliferation and uhrastructure of HUVEC which was induced by rhIL-6. Methods In vitro, HUVEC was cultured and divided into three groups. Group 1 served as controls. Group 2 was given 10 ng/ml rhIL-6. Group 3 was given 10 ng/ml rhIL- 6 ± 10μ mol/Latorvastatin. The changes of proliferous activity and uhrastructure of HUVEC were observed by MTT and transmission electromicroscope. Results ① Compared with the control group, rhIL-6 accelerated the proliferation of HUVEC while the atorvastatin restrained it. The difference was significant.② Compared with the control group, rhIL-6 could increase the amount of ribosomes on rough endoplasmic reticulum, Compared with the rhIL-6 group, atorvastatin reduced the amount of ribosomes. Conclusion Atorvastatin may restrain the effect of cytokines and prevent the proliferation of HUVEC. Along with that effect, the number of ribosomes on rough endoplasmic reticulum was decreased too.

关 键 词：人脐静脉内皮细胞 阿托伐他汀 白细胞介素-6 细胞增殖 超微结构 

分 类 号：R96[医药卫生—药理学]