基于线粒体COⅡ基因的中国蝽科分子系统学研究(半翅目,异翅亚目)  

MOLECULAR SYSTEMATIC STUDY ON PENTATOMIDAE (HEMIPTERA, HETEROPTERA) FROM CHINA BASED ON MITOCHONDRIAL CYTOCHROME OXIDASEⅡ GENE

作　　者：卜云[1] 栾云霞[1] 郑哲民[2] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [2]陕西师范大学动物研究所,西安710062

出　　处：《动物分类学报》2006年第2期239-246,共8页Acta Zootaxonomica Sinica

基　　金：国家自然科学基金资助项目(30370169).

摘　　要：扩增并测定了我国蝽科4亚科8属11种昆虫线粒体COⅡ基因585 bp的序列,对序列的碱基组成、转换颠换、遗传距离等进行分析,探讨了COⅡ基因在该科的分子进化机制.并基于COⅡ基因序列数据,分别采用邻接法（NI）、最大简约法（MP）和贝叶斯推论法（BI）建立蝽科分子系统发育关系.研究结果表明,蝽科昆虫COⅡ基因A＋T含量平均为71.7%,存在较强的A＋T含量偏向性,氨基酸的变异率为27.2%;亚科间的遗传距离介于0.168～0.242之间,大于亚科内属种间的遗传距离,蝽科与盾蝽科2外群之间遗传距离最大,两科之间存在明显的间断.分子系统发育树表明,短喙蝽亚科为蝽科中较为原始的类群,分化较早,益蝽亚科与舌盾蝽亚科关系较近,形成一对姐妹群,蝽科中捕食性种类--益蝽亚科是较为特化的类群,它是由植食性种类分化而来.蝽科4亚科间的分子系统发育关系为Phyllocephalinae＋（Pentatominae＋（Asopinae＋Podopinae）.The mtDNA-CO Ⅱ gene partial sequences （585 bp） of 4 subfamilies 8 genera 11 species of Pentatomidae and 2 species of Scutelleridae （outgroup） were amplified and sequenced. Nucleotides composition, transition and transversion, genetic distance of this segment had being analyzed. We discussed the molecular evolution mechanism of CO Ⅱ gene in Pentatomidae. By using Neighbor-Joining （ NJ ）, Maximum Parsimony （MP） and Bayesian Inference （ BI ） methods, we constructed the molecular phylogeny of Pentatomidae based on CO Ⅱ gene sequence. The results indicate that average A ＋ T content of CO Ⅱ gene in Pentatomidae is 71.7%, which shows a strong A ＋ T bias. The variation ratio of amino acid is 27.2%. Genetic distances among subfamilies （0.168-0.242） are bigger than distances between genera or species of the same subfamily. The distance between Pentatomidae and 2 outgroups of Scutelleridae are the biggest, which reveal a distinct gap between this two families. Molecular phylogenetic trees suggest that Phyllocephalinae is a primordial subfamily of Pentatomidae which is differentiated earlier from other species. Asopinae and Podopinae have close relationship and form a sister group. Asopinae, the predatory insects, is a specialized subfamily differentiated from phytophagous insects. Consequently, the molecular phylogeny of four subfamilies of Pentatomidae is： Phyllocephalinae ＋ （Pentatominae） ＋ （Asopinae ＋ Podopinae）.

关 键 词：半翅目 蝽科 分子系统学 线粒体COⅡ基因 中国. 

分 类 号：Q961[生物学—昆虫学] Q963