复方烫疡油对兔耳瘢痕形成的影响  

Effects of Fu fang Tang Yang You on the formation of proliferation scars of rabbits

作　　者：罗聪[1] 安洪[1] 刘传康[1] 欧云生[1] 杨正钦[2] 

机构地区：[1]重庆医科大学附属第一医院骨科,重庆400016 [2]重庆医科大学药理学教研室,重庆400016

出　　处：《重庆医科大学学报》2006年第3期380-383,458,共5页Journal of Chongqing Medical University

基　　金：重庆大学研究生教育创新基金(2004A028)

摘　　要：目的:观察复方烫疡油(FTY)对兔耳腹侧瘢痕形成的影响,探索其作用机制,为临床应用提供依据。方法:用18只新西兰大白兔耳部腹侧制作创伤模型,于每侧兔耳腹侧作四个创面,每个创面1.2cm×1.0cm切除全层皮肤。获144个创面。将兔随机分为两组(A、B组)。A组全部右耳用FTY治疗;全部左耳用复方烫疡油赋形剂(灭菌的芝麻油)治疗;B组全部右耳用磺胺嘧啶银霜治疗;全部左耳用生理盐水治疗。于创伤后30,60,90天分别取创面组织作组织学观察,并用免疫组织化学法检测Ⅰ、Ⅲ型胶原、转化生长因子-β1(TGF-β1);TUNEL法定量检测组织细胞的凋亡指数。结果:FTY组创面愈合时间较其它组提前约1.5天。创面增殖块中组织中I、III胶原比值、TGF-β1、细胞核增殖抗原(PCNA)表达较其它组低,凋亡指数FTY组明显高于其它组。结论:复方烫疡油不仅能促进创面愈合,而且能减少创面瘢痕形成。Objective： To investigate the effects of Fu Fang Tang Yang You（复方烫疡油）on the formation of proliferation scars at the ventral side of the ear of rabbits and to explore its possible mechanism. Methods： 144 wounds at the ventral side of the ears of 18 New Zealand white rabbits were creatod, The area of each wound was 1.2cm ~ 1.0cm. The wounded rabbits were randomly divided into two groups （groups A and B）. All the wounds on the right ears of the rabbits in group A were treated with FTY; and the wounds on the left with vegetable oil. All the wounds on the right ears of group B were. treated with Sulfadiazine silver cream; and the wounds on the left with norreal saline. Tissues from the wounds were examined respectively. 30d, 60d, 90d after injury. Each sample was immunohistochemically stained and examined TGF-β 1, type of collagen Ⅰ , Ⅲ and proliferating cell nuclear antigen （PCNA） monoclonal antibody were examined.The apoptosis index was calculated with TUNEL technique. Results： In FTY group, the time of wound healing was reduced by about 1.5 days; The expression of TGF- β 1 ,PCNA in the tissues of the wounds was lower than that of the control group. Meanwhile, the apoptoric index of fibreblasts was obviously higher than that of the control group. Conclusion： FTY can promote wound healing with less scar formarion.

关 键 词：大黄素 创伤 瘢痕 愈合 

分 类 号：R619.6[医药卫生—外科学]