26种活血化瘀中药对鸡胚绒毛尿囊膜血管生成的影响  

Effects of 26 drugs for promoting blood circulation and removing blood stasis on the angiogenesis of chick chorioallantoic membrane

作　　者：王淑美[1] 徐晓玉[2] 陈伟海[2] 季业伟[2] 叶兰[2] 

机构地区：[1]重庆医科大学中医药学院,重庆400050 [2]重庆医科大学药学系,重庆400016

出　　处：《重庆医科大学学报》2006年第3期401-403,407,共4页Journal of Chongqing Medical University

基　　金：重庆市科委资助项目渝科发计字[2002]18号-99

摘　　要：目的:采用鸡胚绒毛尿囊膜(ChickChorioallantoicMembrane,CAM)法从26种活血化瘀中药中初筛血管生成抑制剂。方法:试验组分别为26种活血化瘀中药,所用片剂、胶囊剂、丸剂、散剂溶解成饱和溶液,汤剂制成含生药2g/ml的水煎醇沉液,注射液、口服液、膏剂用原药物浓度。种鸡蛋孵化7天,建立CAM模型,按20μl胚加药液于明胶海绵载体上,每日1次,连续3天。孵化第10天,计数CAM载体周围5mm的血管分支点。结果:15种活血化瘀中药CAM的血管分支点数量降低(P<0.05)。结论:榄香烯乳剂、莪术油葡萄糖注射液、磷酸川芎嗪注射液、注射用阿魏酸钠、雷公藤多苷片、火把花根片、祖师麻片、复方斑蝥胶囊、大黄广廿灬虫虫丸、大活络丸、复方丹参滴丸、七厘散、活血通脉片、三棱丸及桃红四物汤Ⅱ号具有抑制CAM血管生成的作用。Objective： To screen angiogenesis inhibitor from 26 drugs for promoting blood circulation and removing blood stasis through chick chorioaUantoic membrane （ CAM ） model in vivo. Methods： The experimental groups were 26 drugs for promoting blood circulation and removing blood stasis. Tablets, capsules, pills and powder of the drugs were adopted in the form of saturated concentration, decoction was in the form of concentration of 2g/ml, and injection, oral liquid and eleetuary were in their own concentration. 7-day-old fertilized white chicken eggs incubated at 37℃ in chamber were prepared by creating a window in the shell. A sponge carrier was placed on CAM and treated with different liquid medicine with the dosage of 20 μ 1 per egg daily for 3 days. On day 10, the number of CAM blood vessel embranchment 5ram around the carrier was counted with a stereomicroscope. Results： 15 drugs for promoting blood circulation and removing blood stasis obviously inhibited the CAM blood vessels （P 〈 0.05）. Conclusion： Elemene Emulsion, Zedoary Turmeric Oil and Glucose injection, Ligustrazine Phosphate injection, Sodium Ferulate for injection, Tripterygium Glycosides, Huoba Huagen tablet, Zushima tablet,Fufang Banmao capsule, Dahuang Zhechong pill, Dahuoluo pill, Qili powder, Huoxue Tongmai tablet, Fufang Danshen drop pill, Shanleng pill and Taohong Siwu decoction Ⅱ have a potential effect on antiangiogenisis.

关 键 词：活血化瘀中药 抗血管生成 鸡胚绒毛尿囊膜 药物筛选 

分 类 号：R285[医药卫生—中药学]