鲍温样丘疹病中hTERT蛋白表达与高危型HPV感染的相关性研究  

Study on the Association between the Expression of hTERT Protein and the Presence of High-risk Human Papillomavirus in Bowenoid Papulosis

作　　者：刘厚君[1] 林能兴[1] 吴艳[1] 黄琨[1] 李家文[1] 古江增隆[2] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,湖北武汉430022 [2]日本九州大学皮肤科

出　　处：《中国皮肤性病学杂志》2006年第5期261-263,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的研究hTERT蛋白在鲍温样丘疹病中的表达及与高危型HPV存在的相关性。方法通过原位杂交法研究HPV的存在和免疫组化法检测hTERT蛋白在鲍温样丘疹病中的表达,并探讨hTERT蛋白表达与高危型HPV存在的相关性。结果在26例鲍温样丘疹病标本中,18例(69.2%)为HPV16/18阳性,其中1例HPV16/18阳性标本也有HPV6/11阳性。3例(11.5%)为HPV31/33/35阳性,2例(7.7%)为未定型HPV阳性,21例有高危型HPV存在的标本是hTERT阳性,16例高危型HPV感染的标本呈现了强而弥漫性hTERT蛋白表达。hTERT蛋白表达与高危型HPV的存在显著相关。结论高危型HPV可能诱导了hTERT蛋白的高表达。Objective To investigate the presence of HPV and expression of p16 and hTERT protein in Bowenoid papulosis （BP） . Methods Biopsy samples of BP from 26 patients were subjected to in situ hybridization for various HPV strains and immunohistochemical staining for hTERT. Results Among the 26 biopsy specimens,in situ hybridization using DNA probes for HPV 16/18 revealed positivity in 18 specimens （69.2%）, one of which also showed positivity with the probes for HPV 6/11. HPV31/ 33/35 was found in 3 specimens （ 11.5 % ）. Two specimens （7.7%） were positive for unclassified HPV. Twenty-one BP specimens that were infected with hrHPV were positive hTERT . Strong and diffuse immunostaining hTERT was seen in 16 hrHPV-infected specimens. The expression of hTERT was significantly associated with the presence of hrHPV. Conclusion hrHPVs were involved in inducing p16 and hTERT overexpression in BP.

关 键 词：HPV 人类端粒酶逆转录酶 鲍温样丘疹病 

分 类 号：R752[医药卫生—皮肤病学与性病学]