原因不明复发性流产患者蜕膜Foxp3 mRNA及蛋白质的表达  

Expressions of Foxp3 mRNA and protein in decidua of women with unexplained recurrent spontaneous abortion.

作　　者：杨卉[1] 林其德[1] 陈广洁 邱丽华[1] 付勤[1] 

机构地区：[1]上海交通大学医学院附属仁济医院妇产科,上海200001 [2]上海市免疫研究所

出　　处：《现代妇产科进展》2006年第4期292-294,F0003,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨原因不明复发性流产(URSA)患者蜕膜Foxp3 mRNA及蛋白的表达。方法:分别采用免疫组化S-P法、半定量和实时定量PCR法和W estern B lot法检测25例URSA患者蜕膜组织Foxp3 mRNA及蛋白的表达与分布,并以32例正常早孕妇女为对照。结果:(1)免疫组化S-P法显示Foxp3表达于胞浆,妊娠后的蜕膜组织有Foxp3的表达;URSA患者蜕膜Foxp3的表达显著低于正常妊娠妇女(P<0.01);(2)半定量和实时定量RT-PCR法分析均显示,URSA患者蜕膜组织Foxp3 mRNA的表达显著低于正常早孕妇女(P<0.05);(3)W estern B lot法分析显示,URSA患者蜕膜组织Foxp3蛋白的表达显著低于正常早孕妇女(P<0.05)。结论:Foxp3可能在维持早期妊娠中起重要作用,其表达水平降低可能与URSA的发病有关。Objective：To observe the expression of Foxp3 mRNA and protein in human decidua of normal pregnant women（NP） and patients with unexplained recurrent spontaneous abortion（URSA）. Method：Expression and distribution of Foxp3 in decidua of 32cases of NP women and 25 cases of URSA patients were observed respectively by immunohistochemistry, semi-quantitative RT-PCR, real-time PCR and Western blotting. Results： （ 1 ） By immunohisto- chemistry, the expression of Foxp3 was observed to be localized in the cytoplasm of pregnancy decidua. Foxp3 expression in URSA patients was lower significantly compared with NP women （ P 〈0. O1 ） ; （2） Expression of Foxp3 mRNA in deciduas of URSA was significantly lower compared with NP women by semi-quantitative RT-PCR and real-time PCR （ P 〈0. 01 ） ; （ 3 ） Expression of Foxp3 protein in deciduas of URSA patients was significantly lower than those of NP women by Western blotting analysis （ P 〈0. 05 ）. Conclusion： The expression of Foxp3 would be an important factor related with the maintenance of pregnancy. The lower expression of Foxp3 in URSA is involved in the pathogenesis of URSA.

关 键 词：流产 复发性 蜕膜 调节性T细胞 

分 类 号：R714.21[医药卫生—妇产科学]