白细胞介素-1受体拮抗剂与子宫内膜异位症的关系  

Research on the Relationship Between Interleukin-1 Receptor Antagonist and Endometriosis

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作  者:温洁[1] 邓琳[1] 张信美[1] 林俊[1] 

机构地区:[1]浙江大学医学院附属妇产科医院,浙江杭州310006

出  处:《浙江预防医学》2006年第6期8-11,共4页Zhejiang Journal of Preventive Medicine

基  金:浙江省自然科学基金项目;项目编号:2002-302756

摘  要:目的探讨白细胞介素1-受体拮抗剂(IL-1ra)蛋白表达量、基因多态性与子宫内膜异位症(EMs)的相关性.方法应用酶联免疫吸附实验对74例EMs患者和68例对照者的血清及腹腔液中IL-1ra蛋白水平进行检测;聚合酶链式反应技术(PCR)扩增138例EMs及100例对照者的IL-1ra基因.结果EMs组血清中IL-1 ra蛋白水平与对照组无差异(P>0.05);而EMs组和对照组腹腔液中IL-1 ra水平中位值分别为102.64和175.18pg/ml,两组比较有显著性差异(P<0.05).其中Ⅲ~Ⅳ期EMs腹腔液中IL-1ra水平低于Ⅰ~Ⅱ期EMs组,并显著低于对照组(P<0.05).IL-1ra基因型A1/A1、A1/A2、A1/A4、A2/A2在EMs患者与对照组的分布频率分别为84.1%、12.3%、2.9%、0.7%和95%、4%、1%、0%,两组比较P<0.05;A1、A2、A4等位基因频率在两组间分别为91.7%、6.9%、1.4%和97.5%、2%、0.5%,具显著性差异(P<0.05).A1/A2基因型个体患EMs的危险性是野生型A1/A1纯合子的3.48倍(95%CI:1.13~10.69),携带A2等位基因者患EMs的危险性是携带A1等位基因者的3.66倍(95%CI:1.23~10.94).结论IL-1ra的蛋白水平在EMs患者尤其是Ⅲ~Ⅳ期EMs者的腹腔液中较对照组显著降低,提示IL-1ra可能参与EMs的发病过程,并和疾病的严重程度有关;且IL-1ra基因的A2等位基因可能是中国妇女患EMs的危险因素之一.Objective To analyze whether the expression level of interleukin-1 receptor antagonist (IL-1Ra) or gene polymorphism of IL-1Ra is associated with endometriosis (EMs). Methods Blood sample and peritoneal fluid were collected from 138 patients with EMs and 100 controls without EMs. The level of IL-1Ra in sertum and peritoneal fluid was detected with ELISA. And the gene of IL-1Ra was amplified with PCR. Results No significant difference was found for IL-1Ra level in serum between two groups (P 〉 0.05). However, the IL-1Ra level in peritoneal fluid of patients with EMs was significantly lower than that of controls, the median is 102.64pg/ml and 175.18pg/ml respectively ( P 〈 0.05). According to staging criteria of r-AFS, the IL-1Ra level in peritoneal fluid of patients with Ⅲ-Ⅳ stage EMs was lower than with Ⅰ -Ⅱ stage EMs and significandy lower than in controls ( P 〈 0.05 ). The frequencies of genotype A 1/A 1, A 1/A2, A1/A4 and A2/A2 for IL- 1Ra were 84.1%, 12.3%, 2.9% and 0.7% in EMs and 95%, 4%, 1% and 0% in controls, respectively (P〈 0.05). The frequencies of A1, A2, and A4 allele were 91.7%, 6.9% and 1.4% in patients and 97.5%, 2%, 0.5% in controls (P 〈 0.05). The variant genotype A 1/A2 significantly increased EMs risk compared with the wild genotype A 1/A 1 (OR = 3.48 95 % CI: 1.13 - 10.69). And allele A2 also increased EMs risk compared with allele A1. (OR=3.66 95%CI: 1.23 - 10.94). Conclusions The IL-1Ra level in EMs, especially in Ⅰ - Ⅱ stage EMs, was significantly lower than that in controls. It suggests that the IL-1Ra may be involved in the pathogenesis of EMs and correlated with the severity of EMs. The A2 allele of IL-1Ra may be one of the risk factors for the Chinese women to suffer EMs.

关 键 词:子宫内膜异位症 白细胞介素-1受体拮抗剂 基因多态性 腹腔液 血清 

分 类 号:R711.71[医药卫生—妇产科学]

 

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