双歧杆菌对NOD小鼠1型糖尿病的免疫干预作用  

Preventive Function of Bifidobacterium for Type I Diabetes in Female NOD Mice

作　　者：张梅[1] 梁国光[2] 李朝品[2] 周丽[3] 

机构地区：[1]安徽理工大学 [2]安徽理工大学医学院 [3]齐齐哈尔医学院中心实验室,161042

出　　处：《齐齐哈尔医学院学报》2006年第4期385-387,共3页Journal of Qiqihar Medical University

摘　　要：目的了解早期应用双歧杆菌对非肥胖糖尿病(NOD)小鼠糖尿病发病的影响。方法将4周龄雌性NOD小鼠随机分为两组:实验组(20只)给予双歧杆菌0.25mg/g体重/天(制备成活菌悬液0.5ml),对照组(20只)给予等量的磷酸盐缓冲液(PBS)。4周龄开始给药至30周龄,每周测体重,于15周龄时各组分别处死6只小鼠,取胰腺组织HE染色进行胰岛炎评分;其余小鼠30周龄时观察发病率,同时观察胰岛炎,脾脏T细胞亚群变化。结果双歧杆菌组糖尿病发病率28.57%,明显低于对照组78.57%(P<0.05),发病时间也显著延缓,胰岛炎程度明显减轻,残存胰岛数目高于对照组;脾CD4+、CD8+T细胞亚群双歧杆菌组明显低于对照组(CD4+T细胞P<0.01,CD8+T细胞P<0.05)。结论双歧杆菌对NOD小鼠1型糖尿病发病有预防和延迟的作用,其机制可能与调节小鼠CD4+、CD8+T细胞亚群有关。Objective To study whether bifidobacterium can prevent the onset of type 1 diabetes in female non-obese diabetic（NOD） mice. Methods 40 female NOD mice aged four weeks were divided into two groups. Experiment group（n= 20）was orally administered bifidobaeterlum（0.25mg/gBM/d, prepared viable organism suspension 0.5ml）,and the contrast group （n= 20） was given PBS by the same regimen. Mice aged four weeks were administered until 30 weeks of age. At the age of fifteen weeks, the degree of insulitis was observed; The incidence of diabetes was dctected at age of thirty weeks, at the same time,to observe insulitis and change of T cell subsets of spleen. Results Treatment with bifidobacterium reduced the total incidence of diabetes（28. 57%Vs 78. 57%, P〈0. 05） and delay its onset compared with control group. The level of insulitis were lessen than control group, but the number of survival areas of Langerhans were higher than control group;CD4^＋ ,CD8^＋ T cell subsets of spleen were lower obviously than control group. （CD4^＋ T cell P〈 0.01 ,CD8^＋ T cell P 〈 0.05）. Conclusions The data demonstrated the preventive effects of bifldobacterium on the onset of type 1 diabetes and the severity of insulitis in female NOD mice. The mechanisms of these effects may be related to the change of CD4^＋ ,CD8^＋ T cell subsets.

关 键 词：双歧杆菌 1型糖尿病 T细胞亚群 

分 类 号：R587.1[医药卫生—内分泌] R735.34[医药卫生—内科学]