与PPAR基因连锁的5个微卫星标记与猪肉质性状的相关性  

Effects of 5 Microsatellite DNA Markers Linked with PPAR Gene on Meat Quality Traits in Pig

作　　者：邵根宝[1] 贾超[1] 刘红林[1] 赵茹茜[1] 丁红梅[2] 陈杰[1] 黄瑞华[2] 徐银学[1] 

机构地区：[1]农业部动物生理生化重点开放实验室,南京210095 [2]南京农业大学动物科技学院,南京210095

出　　处：《农业生物技术学报》2006年第2期162-165,共4页Journal of Agricultural Biotechnology

基　　金：江苏省自然科学基金重点项目(No.BK2002204);国家高技术研究与发展计划(863)项目(No.2002AA242031-3)资助

摘　　要：从已公布的猪13号染色体连锁图谱中选择与PPAR(peroxisomeproliferatoractivatedreceptor)基因连锁的5个微卫星位点,在苏太猪群体中对这些位点进行群体遗传学特性分析。结果表明,各位点等位基因数6￣9个,杂合度0.59￣0.81,多态!"#$0.51￣0.76;背膘厚与嫩度、系水力和肌内脂间的相关系数分别为-0.358、0.020和0.311,且背膘厚与嫩度及肌内脂间的相关性达到极显著水平(P<0.01)。方差分析结果显示,微卫星位点SW482对背膘厚影响显著(P<0.05);SW937对系水力影%达到极显著水平(P<0.01);其它位点S0222、S0281和S0021对各性状影响均未达到显著水平(P>0.05)。The 5 microsatellite DNA loci linked with PPAR （peroxisome proliferator activated receptor） gene were selected from the published genetic map of chromosome 13 in pig, and polymorphisms of these microsatellites in 100 samples from Sutai pigs （Duroc x Erhualian） were detected. The results revealed that the number of alleles were 6--9, heterozygosity 0.59-0.81, polymorphism information content 0.51-0.76, the correlation coefficients between back fat thickness（BFT） and tenderness, water-holding capacity （WHC）, and intmmuscular（IMF） were - 0.358, 0.020 and 0.311, respectively, and there existed significant correlations between BFT and tenderness, IMF （P〈0.01）. Moreover, effects of S0021, SW937, SW482, S0222, and S0281 on meat quality traits were analyzed with PROC GLM of SAS. Results showed that the effect of SW482 on BFT was significant （P〈0.05） and SW937 on WHC reached a significant level at P〈0.01. S0222, S0281 and S0021 had no significant effect on the two selected meat quality traits（P〉0.05）.

关 键 词：微卫星标记 多态性 PPAR基因 肉质性状 

分 类 号：S188[农业科学—农业基础科学]