奥利亚罗非鱼(♀)×鳜(♂)杂交后代及其双亲的染色体核型分析  

Analysis of Chromosome Karyotypes of Oreochromis aurea(♀), Siniperca chuatsi(♂) and Their Offspring

作　　者：曹丽萍[1] 丁炜东[1] 贾永义[1] 夏德全[1] 吴婷婷[1] 

机构地区：[1]中国水产科学院淡水渔业研究中心,无锡214081

出　　处：《农业生物技术学报》2006年第2期187-190,共4页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30371116);国家"十五"攻关专题(No.2004BA526B0110);国家高技术研究与发展计划(863)项目(No.2001AA243061)资助

摘　　要：采用人工授精法以奥利亚罗非鱼(Oreochromisaurea)为母本,鳜(Sinipercaahuasti)为父本,获得了能正常发育的后代。利用微量全血培养法对奥利亚罗非鱼、鳜及它们杂交后代的染色体数目和核型进行了分析,结果表明,奥利亚罗非鱼染色体数!"2n=44,核型公式为6sm+24st+14t,染色体臂数(NF)=50;鳜鱼为2n=48,核型公式为8sm+4st+36t,NF=56;杂交后代为2n=44,核型公式为6sm+26st+12t,NF=50。经对比分析,杂交后代与奥利亚罗非鱼的核型相似。The progenies of the distant hybridization between Oreochromis aurea （ ♀ ） and Siniperca ahuasti （ ♂ ） were successfully obtained through years of research. And their karyotypes were studied by half-micro blood lymphocytic cell cultivation. The result indicated that the diploid chromosome number of O. aurea （ ♀ ） was 2n =44, and karyotype formula was 6sm＋24st＋14t, arm number （NF） =50; the diploid chromosome number of S. chuatsi （ ♂ ） was 2n =48, and karyotype formula was 8sm＋4st＋36t, NF=56; the diploid chromosome number of their progenies was 2n =44, chromosome formula was 6sm＋26st＋12t, NF=50. The results showed the karyotypes between O. aurea （ ♀ ） and progenies were similar by comparison.

关 键 词：奥利亚罗非鱼 鳜 远缘杂交 染色体 

分 类 号：S185[农业科学—农业基础科学]