兔病毒性出血症病毒JX/97株衣壳蛋白基因的序列测定与分析  

Analysis and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus (RHDV) JX/97 Strain

作　　者：刘光清[1] 倪征[1] 张玉颖[1] 云涛[1] 梁华丽[1] 华炯刚[1] 李双茂[1] 

机构地区：[1]浙江省农业科学院农业部病毒学与生物技术重点实验室,杭州310021

出　　处：《农业生物技术学报》2006年第2期191-196,共6页Journal of Agricultural Biotechnology

摘　　要：对中国兔病毒性出血症病毒(Rabbithemorrhagicdiseasevirus,RHDV)JX/97株的衣壳蛋白基因进行了克隆和测序。结果表明,该蛋白的核苷酸序列长度为1739nt,推导的氨基酸序列为579aa。利用分子生物学软件分析了该病毒与国内外22!RHDV的基因序列,结果显示,RHDV不同分离株之间的序列同源性几乎都在90%以上。衣壳蛋白基因的系统发生树分析结果表明,所选的RHDV参考毒株按照一定的遗传距离,可以分为4个基因群,基因群之间的距离有加大的趋势,表明RHDV受环境的影响有向不同方向演变的趋向,即RHDV在长期适应特定环境的过程中,有可能逐渐演变成各具特色的RHDV。The capsid protein gene of Rabbit hemorrhagic disease virus （RHDV） JX/97 strain was cloned and sequenced .The results showed that the nucleotide sequence of the gene was 1 739 nt in length encoding a protein of 580 aa. Variation analysis of the capsid protein gene of JX/97 strains and other 21 published RHDV isolates showed that RHDV had a high degree of conservation, the homology of the amino acids among different isolates exceeded 90%. The results of computer analysis showed that the antigenic region hadn＇ t changed remarkably in past 20 years. The results ofphylogenetic tree analysis showed that all the reference RHDV isolates could be divided into 4 genetic groups and the distant between them appeared to have evolved to different extents, that means during adapting to the special environment, RHDV may form its only biology characteristic gradually.

关 键 词：兔病毒性出血症病毒 衣壳蛋白基因 遗传变异 

分 类 号：S184[农业科学—农业基础科学] S188