烟粉虱B型及二个非B型种群的SCAR分子标记  

SCAR Molecular Markers of the B Biotype and Two Non-B Populations of the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

作　　者：臧连生[1] 江彤[2] 徐婧[1] 刘树生[1] 张友军[3] 

机构地区：[1]浙江大学应用昆虫学研究所 [2]浙江大学生物技术研究所,杭州310029 [3]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《农业生物技术学报》2006年第2期208-212,共5页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展规划(973)计划项目(No.2002CB111403)资助

摘　　要：用随机引物H16对浙江采集的烟粉虱(Bemisiatabaci)B型和2个非B型种群进行RAPD分子标记,结果表明:B型、非B型ZHJ-1和非B型ZHJ-23个种群分别能稳定地扩增出446、390和1317bp的特异性片段。将3个种群的特异性片段分别克隆和测序,根据测序结果设计3对SCAR引物。通过PCR反应条件的优化,B型、非B型ZHJ-1和非B型ZHJ-23个种群各自的SCAR引物分别能扩增出本种群的特异性条带,其大小依次为439、366和1238bp,而其它种群与温室白粉虱(Tri-aleurodesvaporariorum)则无扩增。RAPD was performed with random primer H16 for B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang, China. The results revealed that the specific sequence fragments containing 446, 390 and 1317 nt were amplified for the B biotype, non-B ZHJ-1 and ZHJ-2 populations, respectively. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences. With improvement of the conditions of PCR, the specific fragment ofB biotype, non-B ZHJ-I and non-B ZHJ-2 populations, namely, 439, 366 and 1238 nt, respectively, could be amplified with the SCAR primer of the corresponding population, while the specific fragments of the other populations ofB. tabaci or Trialeurodes vapo. rariorum could not be amplified.

关 键 词：烟粉虱 生物型 种群 RAPD SCAR 分子标记 

分 类 号：S188[农业科学—农业基础科学] S186