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机构地区:[1]北京市海淀区植物组织培养技术实验室,北京100091
出 处:《农业生物技术学报》2006年第2期235-240,i0003,共7页Journal of Agricultural Biotechnology
基 金:北京市科委科技计划项目资助
摘 要:田间发现的韭菜(Alliumtuberosum)无花粉单株经鉴定为雄性不育而雌性可育后,利用组织培养技术将此雄性不育单株迅速扩繁为雄性不育无性系并作为杂交的母本,与其它优良父本杂交,从F1中快速选出了优良品系J-54和新品种海韭一号。雄性不育无性系能长期稳定地试管保存。从3个F1杂种后代和2个品种的后代中选育出无花粉单株,并用组织培养建立了5个相应的雄性不育无性系。A single plant of Allium tuberosum without pollen was discovered in the field and proved to be a male-sterile plant with female fertility. Tissue culture technique was used to multiply the male-sterile plant forming male-sterile clones which were used as female parent crossing with other lines. From the F1 plants, new strain J-54 and new cultivar Haijiu l have been bred. The male-sterile clones could be kept in test tube for a long time. New types of male-sterile plants were also found in self-crossed plants of 3 F1 hybrids and 2 cultivars and 5 new male-sterile clones have been established from them by tissue culture.
关 键 词:韭菜 组织培养 组培快繁 雄性不育无性系 杂交育种
分 类 号:S184[农业科学—农业基础科学]
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