^(60)Co-γ射线对菊花组培苗的诱变效应  

Effect of ^(60)Co-γ Rays on In vitro Mutagenesis of Chrysanthemum morifolium Ramat

作　　者：王晶[1] 刘录祥[1] 赵世荣[1] 郭会君[1] 赵林姝[1] 陈文华[1] 

机构地区：[1]中国农业科学院作物科学研究所农业部农业核技术与航天育种重点开放实验室,北京100081

出　　处：《农业生物技术学报》2006年第2期241-244,i0004,共5页Journal of Agricultural Biotechnology

摘　　要：对菊花(ChrysanthemummorifoliumRamat.)品种秋之山的组培苗,采用5～30Gy的60Co-γ射线照射处理。研究了γ射线对组培苗生长、增殖和生根的影响以及对组培再生植株移栽后生长开花的影响。结果表明20Gy是γ射线处理菊花组培苗的致死剂量;从生根和继代培养生长和增殖的结果看,诱变处理的最佳剂量在10Gy左右;组培再生植株移栽后发现,10Gy处理的植株中花色变异率为8.2%。与对照花鲜艳的纯黄色相比,变异花增加了不同深浅的红色素,且突变植株均为同质稳定突变。The microshoots of in vitro-cultured Chrysanthemum morifolium cv. Qiuzhishan were irradiated with 5-30 Gy ^60Co-γ, rays. After irradiation the microcuttings were transferred to root-inducing medium and subculture medium respectively to study the inductive effects of γ rays on rooting, shoot growth and multiplication on media. The plant growth and flower characters were investigated after the regenerated plantlets were transplanted out of the protected aseptic environment. The results showed that 20 Gy was the lethal dose of ^60Co-γ, rays to irradiate the microshoots of in vitro -cultured Chrysanthemum morifolium cv. Qiuzhishan, while 10 Gy was the optimum dose to induce mutations. After bloom, 8.2% of the plants in the treatment of 10 Gy were selected for their flower color mutations. In contrast with the bright yellow flowers of the control, different shades of red color were obseryed in those mutant flowers. All the mutant plants were homogeneous and stable mutants.

关 键 词：^60Co-γ射线 辐射诱变 菊花 组织培养 

分 类 号：S183[农业科学—农业基础科学] S184