补肾益气活血方含药血清对兔软骨细胞增殖和bFGF-mRNA表达的影响  

The Effect of the Serum in Bushen Yiqi Recipe on the Rabbit Chondrocyte Proliferating and bFGF-mRNA Expressing

作　　者：唐勇[1] 姜杰[1] 孟辉[1] 周永红[1] 孙立[1] 

机构地区：[1]暨南大学医学院,广东广州510632

出　　处：《时珍国医国药》2006年第5期686-687,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广东省中医药管理局资助项目(No.1040077)

摘　　要：目的观察补肾益气活血方对体外培养兔软骨细胞增殖和bFGF-mRNA表达的影响。方法体外分离、培养兔膝骨关节软骨细胞,检测细胞增殖和软骨细胞bFGF-mRNA原位表达。结果补肾益气活血方高、中、低剂量组细胞增殖均优于对照组(P<0.05),软骨细胞bFGF-mRNA表达均显著高于对照组(P<0.01)。结论补肾益气活血方能促进关节炎软骨细胞bFGF-mRNA表达,加强软骨细胞增殖。Objectlve Observing the effect of the serum of Bushen Yiqi Recipe on the rabbit chondrocyte proliferating and bFGF - mRNA expressing. Methods Separating and cultivating the rabbit knee ,chondrocyte in vitro, detecting the chondrocyte proliferation and bFGF -mRNA expressing in situ. Results The results of the chondrocyte proliferation of the high, middle and low dose Bushen Yiqi Recipe group were better than those of the control groups （ P 〈0. 05 ）, the chondrocyte bFGF - mRNA expressing of the high, middle and low dose Bushen Yiqi Recipe group were higher than the control groups （P 〈 0.01 ）. Conclusion Bushen Yiqi Recipe can increase the chondrocyte proliferation by reinforcing the chondrocyte bFGF - mRNA expressing.

关 键 词：补肾益气活血方 软骨细胞 细胞增殖 碱性成纤维细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]