疏肝舒乳颗粒对人乳腺癌MCF-7细胞的毒杀作用研究  

The Cytotoxic Effects of ShuGanShuRu Granules on MCF-7 Human Breast Cancer Cells in vitro

作　　者：王成鑫[1] 王翠兰[1] 赵晓玲[1] 黄萱[1] 

机构地区：[1]江汉大学医学与生命科学学院,430056

出　　处：《时珍国医国药》2006年第5期717-718,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的研究疏肝舒乳颗粒(SGSR)对人乳腺癌MCF-7细胞的细胞毒性作用。方法应用四甲基偶氮唑蓝(MTT)比色法,在试管内研究SGSR对MTTOD值的影响,检测SGSR对MCF-7细胞的毒杀作用。结果SGSR各浓度组光密度值均低于对照组,说明不同浓度的SGSR对MCF-7癌细胞均有一定的杀伤作用。特别是4,1,0.25 g/m l浓度组,其对癌细胞的杀伤率分别达到99.9%,99%,95.55%,差异有显著性(P<0.01)和很好的剂量效应曲线,杀伤率明显高于β-胡萝卜素(β-CA)和SGSR加β-CA浓度组。结论研究结果现显示,S6SR在试管内对MCF-7乳腺癌细胞有明显的毒杀作用,起效机制除了对肿瘤细胞的直接杀伤作用外,其抗突变、抗有丝分裂作用也可导致DNA链断裂,达到干扰基因转录而产生抗癌作用。SGSR不仅可作为治疗乳腺增生性疾病的药物,也可作为乳腺癌放、化疗时的辅助用药。Objectlve To study the cytotoxie effects of ShuGanShuRu Granules （SGSR）on MCF-7 human breast cancer cells in vitro. Methods By using modified MTT assay, the influences of SGSR upon the OD values of MTT were studied and the cytotoxicity of SGSR on MCF-7 human breast cancer cell were monitored. Results The OD values of the different concentrations of SGSR were all lower than that of controlled group, which indicated that all concentrations of SGSR have cytotoxic effects on MCF-7 cells, especially the killing rate of groups of 4, 1 , 0.25g/ml reached 99.9%, 99% and 95.55% respectively. There were statistically striking differences （P 〈0.01 ） and showed good dosage -effect relation. The killer rate is considerably higher than that of β-Carotine（ β-CA ） and β-CA added SGSR groups. Conclusion This study reveals that SGSR has obvious cytotoxic effects on MCF -7 ceils. Its mechanism might be associated with the anti -mutation effects as well as the direct killing activities, thus making DNA chains broken resulting in the death of tumor cells.

关 键 词：疏肝舒乳颗粒 乳腺癌 MCF-7 四甲基偶氮唑蓝法 

分 类 号：R285.5[医药卫生—中药学]