氦-氖激光照射对兔跟腱羟脯氨酸含量的影响  

The effect of He-Ne laser irradiation on the level of hydroxyproline in healing rabbit Achilles tendons

作　　者：李莉[1] 孙俊英[2] 徐岚[3] 

机构地区：[1]苏州大学附属第一人民医院康复医学科,苏州215006 [2]苏州大学附属第一人民医院骨科,苏州215006 [3]苏州大学生物化学教研室

出　　处：《中华物理医学与康复杂志》2006年第5期295-297,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的研究低强度氦-氖(He-Ne)激光照射对跟腱损伤兔的跟腱组织羟脯氨酸(Hyp)含量的影响,并探讨其最佳照射时间。方法选取22只新西兰雄兔,兔龄10-12周,随机分为对照组和激光照射组。对照组4只,跟腱切断缝合手术后不进行激光照射;激光照射组18只,于术后第1天即以低功率He-Ne激光(波长为632．8 nm、功率为18．9 mW)经皮损伤部位光斑扩束照射,每日照射1次,共照射14 d。激光照射组再根据每次照射时间的长短分为激光照射10 min、20 min和30 min亚组,每亚组6只。分别于术后14 d及28 d处死各组实验动物,采集跟腱标本,用Hyp检测试剂盒对Hyp含量进行检测。结果各激光照射组术后14 d Hyp含量均明显高于对照组(P<0．01);激光照射20 min亚组的Hyp含量明显高于激光照射10 min亚组,差异有统计学意义(P<0．01);激光照射20 min亚组术后28 d时Hyp含量较术后14 d明显增高,差异有统计学意义(P<0．01)。结论 He-Ne激光照射可促进兔跟腱组织胶原蛋白的合成,单次照射20 min效果最好。Objective To evaluate the effect of low intensity He-Ne laser irradiation on the level of hydroxyproline in the Achilles tendons of rabbits after experimental tenotomy, and to determine the best irradiation cycle. Methods Twenty-two male New Zealand rabbits, aged 10-12 weeks, were randomly divided into control and experimental groups. The control group received no laser irradiation after tenotomy. The experimental animals were treated with a He-Ne laser （632.8 nm, 18.9 mW） daily for 14 days. The treated animals received 10 minutes, 20 minutes or 30 minutes of irradiation daily. At the 14th day or the 28th day after the operation, the rabbits were sacrificed and the level of hydroxyproline （Hyp） in their Achilles tendons was measured. Results Hyp was significantly higher in all experimental animals than that in the controls. Hyp level in the 20 minutes group was significantly higher than in the 10 minutes group. Hyp level in the 20 minutes group at the 28th day were significantly higher than at the 14th day. Conclusions He-Ne laser irradiation facilitates the synthesis of collagen and enhances the healing of Achilies tendons. The best irradiation time might be 20 minutes.

关 键 词：氦-氖激光 羟脯氨酸 跟腱 

分 类 号：R454.2[医药卫生—治疗学]