高压氧对脑外伤大鼠额叶皮质和海马胆碱乙酰转移酶阳性神经元数目的影响  

Effects of hyperbaric oxygen on choline acetyltransferase in neurons after traumatic brain injury

作　　者：秦杰[1] 尤春景[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院康复医学科,武汉430030

出　　处：《中华物理医学与康复杂志》2006年第5期302-304,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：湖北省卫生厅科研基金项目(No.JXIB044)

摘　　要：目的观察高压氧(HBO)对脑外伤大鼠额叶皮质和海马胆碱乙酰转移酶(ChAT)阳性神经元数目的影响。方法采用自由落体撞击法造模,将30只大鼠随机分为假手术对照组(A组)、脑外伤组(B 组)和脑外伤加HBO治疗组(C组),各组大鼠在造模24 d后同时进行ChAT阳性细胞数及相关参数的定量分析。结果 C组大鼠额叶皮质和海马ChAT阳性神经元数目比B组明显增多(P<0．01)。结论 HBO治疗能抑制乙酰胆碱能神经元的退行性变,从而使损伤大鼠海马区ChAT阳性纤维增生,这可能是HBO治疗改善颅脑损伤大鼠学习记忆功能障碍的原因。Objective To observe the effect of hyperbaric oxygen on choline acetyhransferase ChAT levels in the neurons of rats with traumatic brain injury. Methods Traumatic brain injuries were modeled in rats using Feeney＇s method. Thirty male rats were randomly divided into 3 groups： a traumatic brain injury group （group A） , a traumatic brain injury plus hyperbaric oxygen （HBO） group （group B） , and a sham operation group （group C）. Samples of each rat＇s brain were tested for ChAT using immunohistochemical staining and light microscopy. Resuits ChAT-positive fibers in the hippocampus almost vanished after traumatic brain injury, but positive fibers were markedly increased after HBO treatment. Conclusion HBO treatment can activate neurons in the hippocampus and inhibit regression of cholinergic neurons, which may be one of the mechanisms by which HBO therapy improves learning ability in injured rats.

关 键 词：脑外伤 高压氧 胆碱乙酰转移酶 大鼠 

分 类 号：R651[医药卫生—外科学]