Activation Effect of Cathartic Natural Compound Rhein to CFTR Chloride Channel  

作　　者：SHI Lin-lin XU Li-na HOU Shu-guang LIN Sen YANG Hong MA Tong-hui 

机构地区：[1]Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China [2]Faculty o Life Sciences, Liaoning Normal University, Dalian 116029, P. R. China [3]China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China

出　　处：《Chemical Research in Chinese Universities》2006年第3期312-314,共3页高等学校化学研究（英文版）

基　　金：SupportedbytheNationalNaturalScienceFundforDistinguishedYoungScholars(No.30325011),theNationalNaturalScienceFoundationofChina(Nos.30470405and30570864),DistinguishedYoungScholarsFundofJilinProvince(No.20030112),andExcellentYoungTeachersProgramofMOE,China.

摘　　要：The cystic fibrosis transmembrane conductance regulator （CFFR） is a cAMP-activated chloride channel expressed in intestinal exoerine glands, which plays a key role in intestinal fluid secretion. A natural anthraquinone ac tivator of CFTR Cl^- channel, rhein, was identified by screening 217 single compounds from Chinese herbs via a cellbased halide-sensitive fluorescent assay. Rhein activates CFTR Cl^- transportation in a dose-dependent manner in the presence of cAMP with a physiological concentration. This study provides a novel molecular pharmacological mechanism for the laxative drugs in Treditional Chinese Medicine such as aloe, cascara and senna.The cystic fibrosis transmembrane conductance regulator （CFFR） is a cAMP-activated chloride channel expressed in intestinal exoerine glands, which plays a key role in intestinal fluid secretion. A natural anthraquinone ac tivator of CFTR Cl^- channel, rhein, was identified by screening 217 single compounds from Chinese herbs via a cellbased halide-sensitive fluorescent assay. Rhein activates CFTR Cl^- transportation in a dose-dependent manner in the presence of cAMP with a physiological concentration. This study provides a novel molecular pharmacological mechanism for the laxative drugs in Treditional Chinese Medicine such as aloe, cascara and senna.

关 键 词：CFTR chloride channel ANTHRAQUINONE Laxative drug 

分 类 号：R442.2[医药卫生—诊断学] R975.3[医药卫生—临床医学]