长尾粗叶木根一个新倍半萜内酯的分离(英文)  

A new sesquiterpene lactone from the roots of Lasianthus acuminatissimus

作　　者：李斌[1] 张东明[2] 罗永明[1] 

机构地区：[1]江西中医学院 [2]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2006年第5期426-430,共5页Acta Pharmaceutica Sinica

基　　金：National Natural Science Foundation of China (20262003).

摘　　要：目的研究长尾粗叶木根乙酸乙酯部分中抗类风湿性关节炎的有效成分。方法采用各种色谱方法分离纯化,运用现代波谱技术(1D,2D-NMR和HRMS等)鉴定各种化学成分,并对所分得的化学成分进行抑制β-葡萄糖酸苷酶释放的抗炎活性的筛选。结果从长尾粗叶木乙酸乙酯部分分得8个化合物,分别是:长尾粗叶木内酯A(1)、党参内酯(2)、2,5-二甲氧基-1,4-苯醌(3)、钩藤苷元A(4)、正十九醇(5)、顺-二十二碳-13-烯酸(6)、二十四烷酸(7)和β-谷甾醇(8)。另外,化合物3对PAF刺激的小鼠多形核白细胞β-葡糖苷酸酶的释放具有明显的抑制作用。结论化合物1是自然界首次发现的新化合物,其他化合物均是首次从该属植物中分得,化合物3是长尾粗叶木根的抗炎有效成分。Aim To study the active constituents for the treatment of rheumatoid arthritis from the ethyl acetate extracts of the roots of Lasianthus acuminatissimus Merr. Methods Various chromatographic techniques were used to separate and purify the constituents. Their structures were established on the basis of 1D, 2D NMR and HRMS spectroscopic analyses and their preliminary evaluation of anti-inflammation effect on the release of β-glucuronidase was carried out. Results Eight compounds were isolated and identified as lasianthuslactone A （1） , codonolactone （2）, 2, 5-dimethoxy-1,4-benzoquinone （3） ,uncargenin A （4） , nonadecyl alcohol （5） , 13-docosenoic acid （6） , tetracosanoic acid （7） and β-sitosterol （8）. Compound 3 showed a significant inhibitory effect on release of β-glucuronidase rat polymorphous nuclear leukocytes activated by platelet activating factor （PAF）. Conclusion Compound 1is a new one, the others were isolated from the plant for the first time and 3 is one of active antiinflammation compound in the plant.

关 键 词：长尾粗叶木 长尾粗叶木内酯A 2 5-二甲氧基-1 4-苯醌 抗炎 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]