蛋白芯片技术对自身免疫性抗体的检测及评价  

Sensitivity and speciality of protein chip technology indetection of autoantibody

作　　者：徐华[1] 韩金祥[1] 高雪芹[1] 刘灿兰[2] 李钿[3] 黄海燕[1] 潘继红[1] 

机构地区：[1]山东省医药生物技术研究中心国家卫生部生物技术药物重点实验室,山东济南250062 [2]泰安市中心医院 [3]山东省立医院

出　　处：《山东医药》2006年第14期4-5,共2页Shandong Medical Journal

基　　金：山东省科技厅资助项目(003100104)

摘　　要：目的 探讨蛋白芯片技术检测自身抗体的临床价值。方法 用酶联免疫吸附试验（ELISA）、蛋白芯片法检测抗dsDNA、抗Ro-60／SSA、抗Ro-52／SSA、抗SSB、抗Jo-1、抗磷脂IgG抗体．用间接免疫荧光法（IIF）、蛋白芯片法检测抗核抗体（ANA），分别统计两种方法检测均阳性、均阴性和单阳性的标本数目．并进行评价。结果 芯片法检测ANA的灵敏度为89％、特异度为60％．芯片法对其他6种自身抗体检测的灵敏度为70．0%～87．5%、特异度为92．7％～98．9％。结论 蛋白芯片法检测自身抗体与常规方法比较，特异度较高，有待进一步改善技术提高其灵敏度。Objective： To evaluate the sensitivity and speciality of protein chip technology in the detection of antoantibody and discuss its value in clinical application. Methods： The ANA was detected with indirect immunofluorescence（IIF） and protein chip respectively, and the other six autoantibodies including anti-dsDNA, anti-Ro-60/SSA, anti-Ro-52/SSA, anti-SSB, anti-Jo-1,and anti-phospholipid IgG were simultaneously detected with ELISA and protein chip technology, and numbers of the both positive, both negative and the single positive were analyzed respectively. Results： Compared with IIF in detecting ANA, sensitivity and specificity of the microarray is 89% and 60%; Compared with ELISA in detecting other six autoantibodies, sensitivity and specificity of the microarray is 70. 0% to 87. 5% and 92. 7% to 98. 9%. Conclusion： Compared with routine methods in detecting autoantibodies, protein chip technology has good speciality and its sensitivity should be raised by improving the technology.

关 键 词：蛋白芯片 自身免疫性疾病 自身抗体 间接免疫荧光法 酶联免疫吸附实验 

分 类 号：R446.6[医药卫生—诊断学]