猪圆环病毒Cap蛋白羧基端特异性单克隆抗体的制备  

Preparation oF Special Monoclonal Antibody of Cap Protein Carboxyl Terminus of Porcine Circovirus

作　　者：马贯中[1] 韩凌霞[1] 朴范泽 刘怀然[1] 司昌德[1] 姜骞[1] 曲连东[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,哈尔滨150001 [2]黑龙江八一农业大学动物科技学院

出　　处：《黑龙江八一农垦大学学报》2006年第2期61-64,共4页journal of heilongjiang bayi agricultural university

基　　金：兽医生物技术国家重点实验室开放基金资助项目"猪圆环病毒型特异性单抗的制备"

摘　　要：利用IPTG诱导表达含有猪圆环病毒2型(PCV2)Cap蛋白羧基端421-37nt的重组大肠杆菌pGEX-PCV421-37,经聚丙烯酰胺凝胶电泳(SDS-PAGE)粗分离,免疫BALB/c小鼠,利用PCV2感染PK15细胞筛选PCV2特异性单克隆抗体。共获得5株,腹水的IFA效价范围为1∶1600～1∶8000;除单抗26H5-A3为IgG1亚型外,32A8-G5、33G10-F4、12B5-G3和14E12-A6全部为IgM型;全部轻链都属κ型。The recombinant bacteria pGEX-PCV421-37 which had the protein of Cap with 421-37nt in carboxyl terminus of PCV2 was expressed by using IPTG. Then SDS-PAGE electrophoresis was made to recover the protein by cutting gel method and the protein was immune to BALB/c mouse for monoclonal antibody production. We identified the five mAbs by IFA with PCV2 infected PK-15.The IFA titers of these mAbs were 1：1600-1：8000,in these mAbs 26H5-A3 was IgG1 and 32A8-G5, 33G10-F4、12B5-G3 and 14E12-A6 were IgM, all the light chain of five mAbs were κ.

关 键 词：PCV2 IFA 单抗 

分 类 号：S852.4[农业科学—基础兽医学]