乳牛衣原体病灭活疫苗的研制  

Development of inactivated vaccine against chlamydiosis in dairy cattle

作　　者：邱昌庆[1] 周继章[1] 程淑敏[1] 曹小安[1] 高双娣[1] 贾文孝 

机构地区：[1]中国农业科学院 兰州兽医研究所 家畜疫病病原生物学国家重点实验室 农业部畜禽病毒学重点开放实验室,甘肃兰州730046 [2]陕西省畜牧兽医总站,陕西西安710016

出　　处：《中国兽医科学》2006年第5期343-347,共5页Chinese Veterinary Science

基　　金：国家乳业重大专项(2002BA518A04)中国农业科学院兰州兽医研究所所长基金

摘　　要：将免疫原性稳定的鹦鹉热衣原体SX5菌株灭活,加入油佐剂制备乳牛衣原体病灭活疫苗,并对疫苗的安全性进行了检测。用该疫苗对成年牛和犊牛进行了最小免疫量试验、效力试验和免疫持续期试验;结果表明,最小免疫量分别为3 mL和5 mL,平均免疫保护率达94．4％,免疫持续期达10个月。疫苗保存期试验表明,在4℃条件下可保存12个月。本动物安全试验和田间小试免疫证明,该疫苗安全性好,乳牛注射疫苗后饮食、产乳量及注苗部位没有明显的异常变化。An inactivated oil adjuvant vaccine was developed with the SX5 strain after inactivation and its safety was evaluated. The minimal effective dosages, potency tests and immunity persistent tests of the vaccine for adult dairy cow and calf were performed. The results showed that the minimal effective dosages were 3 mL and 1. 5 mL, respectively, the average protection rate of reached 94.4%, and the immunity persistent period of the vaccine was ten months. The vaccine stored at 4 ℃ for 12 months still was effective as the freshly made one. Safe tests in dairy cattle of various ages and small-scale field trial confirmed that the vaccine was safe, and there were no obviously abnormal changes in appetite, milk production and the site of vaccination.

关 键 词：乳牛衣原体病 灭活疫苗 保护率 安全性 

分 类 号：S852.67[农业科学—基础兽医学]