H5亚型禽流感病毒HA1基因在Sf9昆虫细胞中的表达及其抗原性检测  

Eukaryotic expression of HA1 gene of H5 avian influenza virus in insect cells and the antigenicity of the expressed protein

作　　者：齐岩[1] 孙凌霜[1] 王彬[1] 孙进华[1] 王君伟[1] 师东方[1] 

机构地区：[1]东北农业大学 动物医学院,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2006年第5期352-356,共5页Chinese Veterinary Science

基　　金：国家科技攻关计划项目(2004BA519A23)

摘　　要：利用pBlueBacHis2杆状病毒载体在昆虫细胞Sf9中表达H5亚型禽流感病毒(AIV)的 HA1基因。Western-blotting证明HA1在昆虫细胞中得到了表达,其产物具有抗原性;且不与 H7、H9 AIV的抗血清发生交叉反应,具有良好的特异性。Dot-ELISA结果显示,表达蛋白可以在非变性条件下与相应抗体作用,同样具有良好的抗原性。试验结果证明,表达的H5亚型AIV的 HA1蛋白,为检测禽类体内是否感染H5亚型AIV和监测免疫禽类血清H5抗体水平提供了优良的检测抗原。HA1 gene of H5 avian influenza virus（AIV） was expressed in insect cells by pBlueBacHis2 baculovirus vector. Western-blotting analysis showed that the expressed HA1 protein had antigenicity and did not react with H7 and H9 AIVs anti-serum. Dot-ELISA analysis showed that the expressed recombinant protein had good and special characteristics under non-denaturing conditions. These results demonstrated that the expressed HA1 protein of H5 AIV was a good antigen for detection of H5 AIV infection and for determination of antibody level in vaccinated avian.

关 键 词：禽流感病毒 HA1基因 杆状病毒 真核表达 

分 类 号：S852.659.5[农业科学—基础兽医学] Q786[农业科学—兽医学]