高脂诱导胰岛素抵抗大鼠糖代谢及血抵抗素、脂联素水平的变化  

Changes of glucose metabolism,plasma resistin and adiponectin levels in the insulin-resistant rats induced by high-fat diet

作　　者：方超[1] 杨刚毅[1] 李伶 唐毅 张凌[1] 李钶[1] 李清明 孙勤[1] 舒朝忠 Gunther Boden 

机构地区：[1]重庆医科大学附属第二医院内分泌科,400010 [2]重庆市分子生物学重点实验室,400010 [3]美国Cencotor公司肿瘤研究室,pa19140 [4]美国Temple大学医学院附属医院一般临床研究中心,pa19140

出　　处：《重庆医学》2006年第10期865-867,878,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30270631和30370671);国家教委春晖计划资助项目(2003-56);重庆市教委科研基金资助项目(2004-35);美国NIH基金项目(R01-DK58895;toG.B.)

摘　　要：目的探讨高脂饮食诱导的胰岛素抵抗大鼠糖代谢及抵抗素、脂联素水平的变化。方法采用大鼠扩展胰岛素钳夹和3-3H标记葡萄糖示踪技术,估计高脂诱导胰岛素抵抗(IR)大鼠肝糖及外周组织糖代谢的变化,同时采用酶免法检测血浆抵抗素及脂联素水平。结果高脂喂养组(HF组)大鼠基础血浆游离脂肪酸(FFA)水平明显增高。钳夹稳态时,HF组血浆胰岛素水平明显高于N组,差异有统计学意义(P<0.01),FFA也明显高于N组,差异有统计学意义(P<0.01)。HF组葡萄糖输注率(GIR)较N组减少38%,胰岛素介导的葡萄糖利用率(GRd)较N组亦明显减少21%。N组大鼠肝糖输出(HGP)明显抑制达71%,而在HF组胰岛素介导的HGP的抑制作用受到明显障碍(仅抑制30%)。钳夹结束时,N组血浆抵抗素水平与基础值相比明显降低,差异有统计学意义(P<0.05),而HF组明显高于N组(P<0.05)。两组血浆脂联素水平在钳夹后均明显下降差异有统计学意义(P<0.05)。结论高脂饮食诱导大鼠产生了外周和肝脏的IR。IR的形成可能与血浆FFA升高和在高胰岛素状态下较高的血浆抵抗素水平有关。Objective To investigate the changes of glucose metabolism, plasma resistin and adiponectin levels in insulin-resistant rats induced by high-fat diet. Methods Changes of peripheral and hepatic glucose metabolism in awake rats were evaluated by hyperinsulinemic euglycemic clamp technique combined with 3-3H glucose as a tracer. And plasma resistin and adiponectin were detected by ELISA. Results In baseline, plasma free fatty acids （FFA） levels were increased in high-fat diet rats （HF） compared with normal chow diet rats （N）. During clamp steady-state, plasma insulin and FFA levels were higher in HF group compared with control （P〈0.01）. In HF group, the glucose infusion rate （GIR） and glucose disappearance rate （GRd） were reduced by 38% and 21%, respectively. And the insulin-mediated suppression of hepatic glucose production was up to 71% in N group, but it was impaired and only up to 30% in HF rats （P〈0.01）. At the end of the clamp, plasma resistin levels were decreased in N group and lower than that in HF rats （P〈0.05）. Howevre, plasma adiponectin levels were both decreased after clamp （P〈0.05）. Conclusion High-fat diet induced peripheral and hepatic insulin resistance in rats. It might be associated with elevated FFA and higher plasma resistin levels during hyperinsulinemia.

关 键 词：胰岛素抵抗 糖代谢 抵抗素 脂联素 

分 类 号：R587.1[医药卫生—内分泌]